Cassia alata's dual role in modulating MUC2 expression in Eimeria papillata-infected jejunum and assessing its anti-inflammatory effects

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-06-07 DOI:10.1002/jemt.24628

Rabab E. Elshershaby, Mohamed A. Dkhil, Yasser Dar, Rewaida Abdel-Gaber, Denis Delic, Ibrahim B. Helal

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引用次数: 0

Abstract

Coccidiosis poses significant hazards to animals, particularly in terms of compromised health, reduced productivity, and economic losses in livestock farming. The conventional treatments for coccidiosis often involve synthetic drugs, contributing to concerns about drug resistance and environmental impact. The pressing need for eco-friendly alternatives is highlighted in this study, emphasizing the importance of exploring medicinal plants like Cassia alata leaf extracts (CAE) against Eimeria papillata-induced infection in mice. The CAE exhibited significant phenolic (2.17 ± 0.03 g/100 g) and flavonoid (0.14 ± 0.01 g/100 g) content and demonstrated notable antioxidant activity. In infected mice, the CAE treatment led to a substantial reduction in oocyst output (~6 fold), ameliorating necrotic enteritis and inflammatory changes in the jejunum. Additionally, CAE treatment increased goblet cell numbers (9.3 ± 0.1 / villus) and decreased macrophage infiltration in the intestinal villi. Molecular analyses revealed CAE's positive modulation of MUC2 gene and notably reduced the levels of pro-inflammatory cytokines (specifically IL-1β, IL-10, and IFN-γ) when contrasted with the infected cohort. Furthermore, CAE treatment significantly reduced nitric oxide levels (44.03 ± 2.4 μmol/mg), showcasing its anti-inflammatory properties. The findings of this study not only contribute to the understanding of CAE's therapeutic potential but also underscore the importance of seeking eco-friendly alternatives in the face of coccidiosis challenges, addressing both the well-being of animals and the sustainability of agricultural practices.

Research Highlights

Cassia alata extract (CAE) exhibited significant phenolic and flavonoid content, displaying notable antioxidant activity.
In infected mice, CAE treatment led to a substantial reduction in oocyst output, ameliorating necrotic enteritis and inflammatory changes in the jejunum.
CAE treatment increased goblet cell numbers and decreased macrophage infiltration in the intestinal villi, while molecular analyses revealed its positive modulation of the MUC2 gene and notable reduction in pro-inflammatory cytokine levels.
Additionally, CAE treatment significantly reduced nitric oxide levels, showcasing its anti-inflammatory properties.

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决明子在调节乳头状艾美耳疫病空肠中 MUC2 表达和评估其抗炎作用方面的双重作用

球虫病对动物危害极大，尤其是在损害健康、降低生产力和造成畜牧业经济损失方面。球虫病的传统治疗方法通常使用合成药物，从而引发了对耐药性和环境影响的担忧。本研究强调了对生态友好型替代品的迫切需要，并强调了探索决明子叶提取物（CAE）等药用植物对乳头状艾美耳菌诱导的小鼠感染的重要性。决明子叶提取物的酚类（2.17 ± 0.03 克/100 克）和类黄酮（0.14 ± 0.01 克/100 克）含量很高，并具有显著的抗氧化活性。在受感染的小鼠中，CAE 处理可大幅减少卵囊产量（约 6 倍），改善坏死性肠炎和空肠炎症变化。此外，CAE 还能增加肠绒毛中的鹅口疮细胞数量（9.3 ± 0.1 个/绒毛）并减少巨噬细胞的浸润。分子分析表明，CAE 对 MUC2 基因有积极的调节作用，并显著降低了促炎细胞因子（特别是 IL-1β、IL-10 和 IFN-γ）的水平。此外，CAE 治疗还能明显降低一氧化氮水平（44.03 ± 2.4 μmol/mg），显示出其抗炎特性。这项研究的结果不仅有助于人们了解 CAE 的治疗潜力，而且还强调了在面临球虫病挑战时寻求生态友好型替代品的重要性，这既有利于动物的健康，也有利于农业实践的可持续发展。研究亮点：决明子提取物（CAE）含有大量酚类和类黄酮，具有显著的抗氧化活性。在受感染的小鼠体内，CAE 可显著减少卵囊的排出量，改善坏死性肠炎和空肠的炎症变化。CAE 治疗增加了肠绒毛中腺泡细胞的数量，减少了巨噬细胞的浸润，而分子分析表明，CAE 对 MUC2 基因有积极的调节作用，并显著降低了促炎细胞因子的水平。此外，CAE 还能显著降低一氧化氮水平，从而显示出其抗炎特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464