Streptococcus dentisani inhibits the growth of Candida albicans and Candida glabrata: in vitro assay.

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Microbiology Pub Date : 2024-06-07 DOI:10.1007/s10123-024-00525-7

Juan Gerardo Sánchez Medina, José Luis Cuéllar Camacho, Jaime Ruiz Garcia, Alex Mira, Rita Elizabeth Martínez Martínez, Mauricio Comas-García, Arturo Garrocho Rangel, Amaury Pozos-Guillén, Saray Aranda Romo

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引用次数: 0

Abstract

Background: Probiotic bacteria inhibit aggregation, biofilm formation, and dimorphism of Candida spp. However, the effects of a new probiotic, Streptococcus dentisani, on the growth of Candida albicans and Candida glabrata biofilms are unknown.

Objective: To determine the effect of S. dentisani on the different phases of C. albicans and C. glabrata biofilm development.

Methods: Growth quantification and ultrastructural analyses were performed on biofilms of C. albicans ATCC 90028, C. glabrata ATCC 2001, and clinical isolates of C. albicans from oral candidiasis (CA-C1), caries (CA-CR1), and periodontal pocket (CA-P1) treated with cell suspensions of S. dentisani CECT 7746. Cell viability was determined by quantifying colony-forming units (CFU/mL). The ultrastructural analyses were done with atomic force microscopy.

Results: S. dentisani induced a significant reduction (p < 0.05) of CFU/mL of immature and mature biofilm in all strains of C. albicans and C. glabrata. Microscopic analysis revealed that S. dentisani reduced C. albicans density in mixed biofilm. The fungus-bacteria interaction affected cell membrane integrity in yeast.

Conclusion: For the first time, our data elucidate the antifungal effect of S. dentisani on the development of C. albicans and C. glabrata biofilms, supporting its usefulness as a niche-specific probiotic to prevent and treat oral dysbiosis.

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牙链球菌抑制白色念珠菌和光滑念珠菌的生长：体外试验。

背景：然而，一种新型益生菌--牙链球菌对白念珠菌和胶状念珠菌生物膜生长的影响尚不清楚：目的：确定牙链球菌对白念珠菌和胶状念珠菌生物膜生长不同阶段的影响：方法：对白僵菌 ATCC 90028、草履虫 ATCC 2001 和来自口腔念珠菌病（CA-C1）、龋齿（CA-CR1）和牙周袋（CA-P1）的白僵菌临床分离株的生物膜进行生长定量和超微结构分析。细胞活力通过菌落形成单位（CFU/mL）的定量来确定。用原子力显微镜进行超微结构分析：结果：S. dentisani诱导的细胞数量显著减少（p 结论：S. dentisani诱导的细胞数量显著减少：我们的数据首次阐明了牙孢子菌对白僵菌和草履虫生物膜的发展具有抗真菌作用，支持其作为预防和治疗口腔菌群失调的特异性益生菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Microbiology 生物-生物工程与应用微生物

CiteScore

5.50

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials. A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.