Histological, biochemical and immunohistochemical assessments of Roundup®, atrazine, and 2,4-D mixtures on tissue architecture, body fluid conditions, nitrotyrosine protein and Na+/K+-ATPase expressions in the American oyster, Crassostera virginica

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Asif Ahmed , Md Saydur Rahman
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引用次数: 0

Abstract

Pesticides are widely used to control weeds and pests in agricultural settings but harm non-target aquatic organisms. In this study, our objective was to evaluate the effect of short-term exposure (one week) to environmentally relevant concentrations of pesticides mixture (low concentration: 0.4 μg/l atrazine, 0.5 μg/l Roundup®, and 0.5 μg/l 2,4-D; high concentration: 0.8 μg/l atrazine, 1 μg/l Roundup®, and 1 μg/l 2,4-D) on tissue architecture, body fluid conditions, and 3-nitrotyrosine protein (NTP) and Na+/K+-ATPase, expressions in tissues of American oyster (Crassostrea virginica) under controlled laboratory conditions. Histological analysis demonstrated the atrophy in the gills and digestive glands of oysters exposed to pesticides mixture. Periodic acid-Schiff (PAS) staining showed the number of hemocytes in connective tissue increased in low- and high-concentration pesticides exposure groups. However, pesticides treatment significantly (P < 0.05) decreased the amount of mucous secretion in the gills and digestive glands of oysters. The extrapallial fluid (i.e., body fluid) protein concentrations and glucose levels were dropped significantly (P < 0.05) in oysters exposed to high-concentration pesticides exposure groups. Moreover, immunohistochemical analysis showed significant upregulations of NTP and Na+/K+-ATPase expressions in the gills and digestive glands in pesticides exposure groups. Our results suggest that exposure to environmentally relevant pesticides mixture causes morphological changes in tissues and alters body fluid conditions and NTP and Na+/K+-ATPase expressions in tissues, which may lead to impaired physiological functions in oysters.

Abstract Image

Roundup®、阿特拉津和 2,4-D 混合物对美国牡蛎(Crassostera virginica)组织结构、体液状况、硝基酪氨酸蛋白和 Na+/K+-ATPase 表达的组织学、生物化学和免疫组织化学评估。
杀虫剂被广泛用于控制农业环境中的杂草和害虫,但会对非目标水生生物造成危害。在这项研究中,我们的目标是评估短期(一周)接触环境相关浓度的农药混合物(低浓度:0.4 μg/L 阿特拉津、0.5 μg/L Roundup0.4 μg/L atrazine、0.5 μg/L Roundup® 和 0.5 μg/L 2,4-D;高浓度:在受控实验室条件下,对美国牡蛎(Crassostrea virginica)的组织结构、体液状况、3-硝基酪氨酸蛋白(NTP)和 Na+/K+-ATPase 的表达进行分析。组织学分析表明,暴露于农药混合物的牡蛎的鳃和消化腺出现萎缩。过期酸-Schiff(PAS)染色显示,结缔组织中的血细胞数量在低浓度和高浓度农药接触组中都有所增加。然而,农药处理明显增加了农药暴露组鳃和消化腺中 P +/K+-ATPase 的表达量。我们的研究结果表明,暴露于环境相关的农药混合物会引起组织形态变化,改变体液状况和组织中 NTP 和 Na+/K+-ATPase 的表达,从而可能导致牡蛎生理功能受损。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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