Metagenomic Analysis of Biliary Microbial Flora in Patients with Gallbladder Cancer or Gallstones-Associated Chronic Cholecystitis.

IF 1.8 4区医学 Q3 ONCOLOGY

Cancer Investigation Pub Date : 2024-07-01 Epub Date: 2024-06-07 DOI:10.1080/07357907.2024.2361305

Ratnakar Shukla, Yasuo Tsuchiya, Anu Behari, Toshikazu Ikoma, Kazutoshi Nakamura, Vinay K Kapoor

引用次数: 0

Abstract

Biliary dysbiosis is associated with gallbladder cancer (GBC). We aimed to look for biliary bacteria specifically detected in GBC patients. We used 16S rRNA-based metagenomic analysis to elucidate biliary microbiota in 30 GBC and 30 gallstones-associated chronic cholecystitis patients. Relative abundance of five genera, Streptococcus, Enterococcus, Halomonas, Escherichia and Caulobacter was significantly associated with GBC. Of 15-species, 7 were detected significantly higher in GBC, Streptococcus anginosus, Streptococcus constellatus, Streptococcus intermedius, Actinomyces bowdenii, Actinomyces israelii, Actinomyces gerencseriae, and Escherichia fergusonii were biosafety level-2 infectious bacteria; other 8 species were biosafety level-1 bacteria. These bacterial species may be involved in pathogenesis of GBC.

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胆囊癌或胆结石相关慢性胆囊炎患者胆道微生物菌群的元基因组分析

胆道菌群失调与胆囊癌（GBC）有关。我们的目的是寻找在 GBC 患者中特异性检测到的胆道细菌。我们使用基于 16S rRNA 的元基因组分析来阐明 30 名 GBC 和 30 名胆结石相关慢性胆囊炎患者的胆道微生物群。链球菌、肠球菌、卤单胞菌、埃希氏菌和球菌这五个菌属的相对丰度与 GBC 有显著相关性。在 15 个菌种中，有 7 个菌种在 GBC 中的检出率明显较高，其中安吉诺斯链球菌、星座链球菌、中间链球菌、鲍登放线菌、以色列放线菌、革兰氏放线菌和弗格森大肠杆菌为生物安全二级感染性细菌；其他 8 个菌种为生物安全一级细菌。这些细菌可能与 GBC 的发病机制有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Investigation 医学-肿瘤学

CiteScore

3.80

自引率

4.20%

发文量

审稿时长

8.5 months

期刊介绍： Cancer Investigation is one of the most highly regarded and recognized journals in the field of basic and clinical oncology. It is designed to give physicians a comprehensive resource on the current state of progress in the cancer field as well as a broad background of reliable information necessary for effective decision making. In addition to presenting original papers of fundamental significance, it also publishes reviews, essays, specialized presentations of controversies, considerations of new technologies and their applications to specific laboratory problems, discussions of public issues, miniseries on major topics, new and experimental drugs and therapies, and an innovative letters to the editor section. One of the unique features of the journal is its departmentalized editorial sections reporting on more than 30 subject categories covering the broad spectrum of specialized areas that together comprise the field of oncology. Edited by leading physicians and research scientists, these sections make Cancer Investigation the prime resource for clinicians seeking to make sense of the sometimes-overwhelming amount of information available throughout the field. In addition to its peer-reviewed clinical research, the journal also features translational studies that bridge the gap between the laboratory and the clinic.