A novel coupling process to replace the traditional multi-stage anammox process—sulfur autotrophic denitrification coupled anammox system

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2024-06-06 DOI:10.1007/s10532-024-10077-2

Sai Yao, Kuo Zhang, Song Yang, Zijun Li, Youzhao Wang, Feng Ma, Pu Chen, Tong Zhu

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Abstract

A novel coupling process to replace the traditional multi-stage anammox process—sulfur autotrophic denitrification (SAD) coupled anaerobic ammonium oxidation (anammox) system was designed, which solved problems of nitrate produced in anammox process and low nitrate conversion rate caused by nitrite accumulation in SAD process. Different filter structures (SAD filter and anammox granular sludge) were investigated to further explore the excellent performance of the novel integrated reactor. The results of sequential batch experiments indicated that nitrite accumulation occurred during SAD, which inhibited the conversion of nitrate to dinitrogen gas. When SAD filter and anammox granular sludge were added to packed bed reactor simultaneously, the nitrate removal rate increased by 37.21% and effluent nitrite concentration decreased by 100% compared to that achieved using SAD. The stratified filter structure solved groove flow. Different proportion influence of SAD filter and anammox granular sludge on the stratified filter structure was evaluated. More suitable ratio of SAD filter to anammox granular sludge was 2:1. Proteobacteria (57.26%), Bacteroidetes (20.12%) and Chloroflexi (9.95%) were the main phyla. The dominant genera of denitrification functional bacteria were Thiobacillus (39.80%), Chlorobaculum (3.99%), norank_f_PHOs-HE36 (2.90%) and Ignavibacterium (2.64%). The dominant genus of anammox bacterium was Candidatus_Kuenenia (3.05%).

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取代传统多级氨氧化工艺的新型耦合工艺--硫自养反硝化耦合氨氧化系统。

设计了一种替代传统多级厌氧工艺的新型耦合工艺--硫自养反硝化（SAD）耦合厌氧氨氧化（anammox）系统，解决了anammox工艺产生硝酸盐和SAD工艺亚硝酸盐积累导致硝酸盐转化率低的问题。研究了不同的过滤结构（SAD 过滤器和厌氧颗粒污泥），以进一步探索新型集成反应器的优异性能。连续批次实验结果表明，在 SAD 过程中会出现亚硝酸盐积累，从而抑制硝酸盐向二氮气的转化。在填料床反应器中同时加入 SAD 过滤器和厌氧颗粒污泥后，硝酸盐去除率提高了 37.21%，出水亚硝酸盐浓度比使用 SAD 时降低了 100%。分层过滤结构解决了槽流问题。评估了 SAD 过滤器和厌氧颗粒污泥的不同比例对分层过滤器结构的影响。SAD 过滤器与厌氧颗粒污泥的比例为 2:1。主要的反硝化菌属有蛋白菌（57.26%）、类杆菌（20.12%）和绿藻菌（9.95%）。反硝化功能细菌的优势菌属为硫杆菌（39.80%）、绿杆菌（3.99%）、norank_f_PHOs-HE36（2.90%）和伊格纳维氏菌（2.64%）。主要的厌氧菌属是库恩尼菌（Candidatus_Kuenenia）（3.05%）。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.