Hepatic immune response of Coilia nasus infected with Anisakidae during ovarian development

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Congping Ying , Zhong Hua , Fengjiao Ma , Yanping Yang , Yinping Wang , Kai Liu , Guojun Yin
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Abstract

Anisakidae parasitism is a prevalent disease in wild populations of Coilia nasus, and can result in a significant loss of germplasm resources. To elucidate the immune response mechanism of C. nasus livers to Anisakidae infection, we collected and analysed 18 parasitic and 18 non-parasitic livers at gonadal developmental stages II, III, and V using histopathology, molecular biology and transcriptome methods. The hepatic portal area of the parasitic group exhibited an increase in the fibrous stroma and thickened hepatic arteries with positive Ly-6G staining, indicating inflammation and immune responses in the liver. Hepatocyte cytokine levels and the expression of liver function-related genes indicated that fish livers responded similarly to Anisakidae parasitism across different gonadal developmental stages. Oxidative stress indices showed more intense changes in stage II samples, whereas gene expression levels of Nrf2 and C3 were significantly increased in parasitised livers during stage III and V. Liver transcriptome sequencing identified 2575 differentially expressed genes between the parasitic and non-parasitic groups at the three gonadal developmental stages. KEGG pathway analysis showed that natural killer cell-mediated cytotoxicity, the NOD-like receptor signaling pathway, neutrophil extracellular trap formation, and other immune pathways were significantly enriched. Expression patterns varied across developmental stages, suggesting that innate immunity was primarily responsible for the liver immune response to Anisakidae infection during C. nasus migration, possibly related to water temperature changes or shifts in the gonadal developmental stage. In summary, this study investigated the immune response of C. nasus to Anisakidae parasitism under natural conditions, focusing on reproductive aspects and environmental changes, thereby establishing a foundation for elucidating the molecular mechanisms underlying the immune response of Anisakidae in C. nasus.

Abstract Image

卵巢发育过程中鲚鱼感染 Anisakidae 的肝脏免疫反应
Anisakidae 寄生虫病是鼻鲚野生种群中的一种流行病,可导致种质资源的大量损失。为了阐明鼻鲚肝脏对Anisakidae感染的免疫反应机制,我们收集并使用组织病理学、分子生物学和转录组学方法分析了18个寄生和18个非寄生肝脏,这些肝脏处于性腺发育阶段II、III和V。寄生虫组的肝门区纤维基质增加,肝动脉增粗,Ly-6G染色阳性,表明肝脏存在炎症和免疫反应。肝细胞细胞因子水平和肝功能相关基因的表达表明,不同性腺发育阶段的鱼类肝脏对 Anisakidae 寄生虫的反应相似。肝脏转录组测序发现,在三个性腺发育阶段,寄生组和非寄生组之间有 2575 个差异表达基因。KEGG 通路分析表明,自然杀伤细胞介导的细胞毒性、NOD 样受体信号通路、中性粒细胞胞外捕获器的形成以及其他免疫通路被显著富集。不同发育阶段的表达模式各不相同,表明先天性免疫主要负责鼻疽梭鱼洄游过程中肝脏对Anisakidae感染的免疫反应,这可能与水温变化或性腺发育阶段的转变有关。总之,本研究研究了自然条件下鼻鲚对Anisakidae寄生的免疫反应,重点研究了鼻鲚的繁殖和环境变化,从而为阐明鼻鲚对Anisakidae免疫反应的分子机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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