Mitochondria-targeting phenothiazine-based ratiometric fluorescent probe for visual and rapid detection of hypochlorous acid in living cells and zebrafish
Fan Yang , Xinlei Song , Mei Zhang , Hongcai Ma , Sai Zhang , Wei Wang , Rui Wang , Zengkai Wang , Zhongxuan Yuan , Dongyin Ren , Wan Sun
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引用次数: 0
Abstract
Hypochlorous acid (HClO) as a reactive oxygen species (ROS) plays an important role in many pathological and physiological processes. Abnormal concentrations of HClO in vivo have close contact with many diseases, including inflammatory diseases and cancer. Due to the measurement deviation caused by changes in external factors, a ratiometric fluorescent probe can effectively avoid this phenomenon. In this work, a ratiometric fluorescent probe PTB was synthesized based on phenothiazine for rapid and specific detection of HClO. PTB showed good selectivity, good biocompatibility, and a low detection limit (21.4 nM). Moreover, this ratiometric fluorescent nanoprobe was subsequently developed for visual on-site detection of HClO with the naked eyes under an ultraviolet lamp. In biology, PTB showed good mitochondrial targeting and was capable of rapidly detecting endogenous HClO within 10 s in living cells and in vivo, which makes it have broad application potential in the life sciences.
期刊介绍:
Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied.
Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media.
The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.