A synergistic effect of herb and acupuncture on the methamphetamine

IF 2.8 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Integrative Medicine Research Pub Date : 2024-06-01 DOI:10.1016/j.imr.2024.101052

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引用次数: 0

Abstract

Background

Herbal medicine Ja-Geum-Jeong (JGJ) has been used for the treatment of detoxification in Eastern Asia. However, the mechanisms involved are not clearly defined. The purpose of the present study was to investigate if herb medication inhibits Methamphetamine (METH)’s reinforcing effect and also examined if a combination of herb medication and acupuncture produces a synergistic effect on METH.

Methods

Male Sprague-Dawley rats were given acute METH intraperitoneally and the locomotor activity and ultrasonic vocalization (USV) calls were measured. Rats were administered JGJ orally and acupuncture was given at HT7 or SI5. Monosodium glutamate (MSG) and gamma-aminobutyric acid (GABA) agonists were injected into the Central amygdala (CeA) to investigate a possible neuroscientific mechanism. Tyrosine hydroxylase (TH) and fast scan cyclic voltammetry (FSCV) were measured to immunohistochemically and electrically confirm the behavioral data.

Results

Locomotor activity and USV calls were increased by METH (P < 0.05) and these increases were inhibited by JGJ (P < 0.05). Also, JGJ had no effect on the normal group given saline, and acupuncture at SI5 acupoint, but not at HT7 acupoint, produced a synergistic effect when combined with JGJ (P < 0.05). The JGJ's inhibition was blocked by the inactivation of CeA (P < 0.05), and MSG mimicked JGJ (P < 0.05). TH and FSCV measures showed the same pattern with the behavioral data (P < 0.05).

Conclusion

Results of the present study suggest that JGJ had inhibitory effects on the METH which was mediated through the activation of CeA and that combination of acupuncture and herb produced synergistic effect.

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中草药和针灸对甲基苯丙胺的协同作用

背景在东亚，草药 Ja-Geum-Jeong (JGJ) 一直被用于解毒治疗。然而，其中的机制尚不明确。本研究的目的是探讨中草药是否能抑制甲基苯丙胺（METH）的强化作用，以及中草药与针灸的结合是否能对 METH 产生协同作用。大鼠口服 JGJ 并针刺 HT7 或 SI5。向中央杏仁核（CeA）注射谷氨酸钠（MSG）和γ-氨基丁酸（GABA）激动剂，以研究可能的神经科学机制。对酪氨酸羟化酶（TH）和快速扫描循环伏安法（FSCV）进行了测量，以免疫组织化学和电学方法证实行为数据。结果METH增加了运动活动和USV呼叫（P< 0.05），JGJ抑制了这些增加（P< 0.05）。此外，JGJ 对给予生理盐水的正常组没有影响，而针刺 SI5 穴位（而不是 HT7 穴位）与 JGJ 联合使用会产生协同效应（P < 0.05）。CeA的失活阻断了JGJ的抑制作用（P <0.05），味精模拟了JGJ（P <0.05）。结论本研究结果表明，JGJ 对 METH 有抑制作用，这种抑制作用是通过激活 CeA 介导的，针灸与中草药的结合产生了协同效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Integrative Medicine Research Medicine-Complementary and Alternative Medicine

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

12 weeks

期刊介绍： Integrative Medicine Research (IMR) is a quarterly, peer-reviewed journal focused on scientific research for integrative medicine including traditional medicine (emphasis on acupuncture and herbal medicine), complementary and alternative medicine, and systems medicine. The journal includes papers on basic research, clinical research, methodology, theory, computational analysis and modelling, topical reviews, medical history, education and policy based on physiology, pathology, diagnosis and the systems approach in the field of integrative medicine.