Temporal fiber-optic fluorescence study of FAD-NADH interactions in phantoms for potential applications in rapid liquid optical biopsy procedures

Q3 Physics and Astronomy

Results in Optics Pub Date : 2024-06-01 DOI:10.1016/j.rio.2024.100707

Subitcha Jayasankar, Sujatha Narayanan Unni

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引用次数: 0

Abstract

Body fluids carry several biomarkers and genetic material that enable the detection of carcinogenesis and tumor metastasis. Owing to this, liquid biopsy is gaining importance and entering clinical practice as an adjunct modality to the current standard of practice in cancer detection. Liquid biopsy involving specimens such as urine is non-invasive and has biomarkers reflecting the tumor microenvironment. We investigate the normal and altered levels of cellular metabolic by-products in liquid phantoms and demonstrate their photon-induced excited-state interaction behaviour for potential diagnostic applications. Short-span temporal analysis of metabolic by-products FAD and NADH in liquid phantoms with fiber optic fluorescence spectroscopy is carried out. The mixed liquid phantom of FAD and NADH, showed excited state interactions that manifested as simultaneous inverse variation in the fluorescence emission from FAD and NADH in varying pH environments. Linear discriminant analysis of the time-varying integrated fluorescence intensity resulted in a 100% accuracy in classifying liquid samples between high and low metabolite concentrations. An extension of application of the experiment model for analysis of body fluids such as urine is also discussed.

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在模型中对 FAD-NADH 相互作用进行时态光纤荧光研究，有望应用于快速液体光学活检程序

体液中含有多种生物标记物和遗传物质，可用于检测癌变和肿瘤转移。因此，液体活检作为目前癌症检测标准的一种辅助方式，正日益受到重视并进入临床实践。涉及尿液等标本的液体活检是非侵入性的，具有反映肿瘤微环境的生物标记物。我们研究了液体模型中细胞代谢副产物的正常水平和变化水平，并展示了它们在光子诱导下的激发态相互作用行为，以用于潜在的诊断应用。我们利用光纤荧光光谱对液态模型中的代谢副产物 FAD 和 NADH 进行了短时分析。FAD 和 NADH 混合液体模型显示出激发态相互作用，表现为 FAD 和 NADH 在不同 pH 值环境下的荧光发射同时发生反向变化。通过对随时间变化的综合荧光强度进行线性判别分析，对高浓度和低浓度代谢物液体样本进行分类的准确率达到了 100%。此外，还讨论了实验模型在尿液等体液分析中的扩展应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Results in Optics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

2.50

自引率

0.00%

发文量

115

审稿时长

71 days