Recent rogress in dual/multi-modal detection modes for improving sensitivity and specificity of lateral flow immunoassays applied for point-of-care diagnostics
Partha Chowdhury , Richardson Lawrance , Zhao-Yu Lu , Hong-Cheu Lin , Yang-Hsiang Chan
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引用次数: 0
Abstract
During the COVID-19 pandemic, lateral flow assay (LFA) based diagnostics witnessed an unprecedented expansion, enabling testing access beyond the conventional healthcare system. Their appeal lies in their user-friendly nature, affordability, and swift results. However, their impact on disease control is limited by factors like sensitivity and specificity. To address these issues and achieve high accuracy, affordability, PCR-level sensitivity, and rapid detection, significant progress has been made in the development of next-generation lateral flow test strips. This review primarily focuses on dual/multi-modal immunoassay techniques, extensively employed to enhance LFA diagnostic sensitivity. Additionally, we delve into the separate roles of dual and multi-modal readers in ensuring specificity and amplifying signal sensitivity. Finally, the challenges and potential future outlook are thoroughly explored for advancing diagnostic methods.
期刊介绍:
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.