Physical Modifications of Kombucha-Derived Bacterial Nanocellulose: Toward a Functional Bionanocomposite Platform

Abstract

Sustainable functionalization of bacterial cellulose for cost-effective bionanocomposites with desired properties has received growing attention in recent years. This article presents the results of work aimed at obtaining bionanocomposite materials based on bacterial cellulose, a natural and eco-friendly material. Bacterial cellulose obtained from the Kombucha symbiotic culture of bacteria and yeast (SCOBY) fermentation process is functionalized by embedding with diatom frustules, silver nanoparticles (AgNPs), and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS). The effects of functionalization on mechanical, optical, plasmonic, electrical, chemiluminescent, and antimicrobial properties are evaluated. Morphological characteristics of the nanocomposites are studied using electron microscopy. Addition of diatom frustules introduced into the SCOBY culture media results in bionanocomposite materials with enhanced tensile strength and increased ultraviolet (UV) blockage properties. In situ functionalization of bacterial cellulose with AgNPs tunes plasmonic and chemiluminescent properties, revealing the biosensing potential of the material. Modified bacterial cellulose shows antimicrobial activity in experiments with gram-positive and gram-negative bacteria. Dual functionalization of bacterial cellulose with PEDOT:PSS and AgNPs results in improved electrical conductivity of the bionanocomposite. Overall, bottom-up physical functionalization approaches and the resulting bionanocomposite materials will open up new opportunities for the low-cost production of green materials and contribute to the development of a sustainable economy.