Circ_0084615 promotes epithelial-mesenchymal transition-mediated tumor progression in hepatocellular carcinoma

IF 2.9 4区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Annals of Gastroenterological Surgery Pub Date : 2024-06-03 DOI:10.1002/ags3.12828

Yu Wu, Li Peng

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Abstract

Aim

CircRNAs have been identified as crucial regulators in tumorigenesis and progression. This study aimed to explore the biological role and underlying mechanism of circ_0084615 in hepatocellular carcinoma (HCC).

Methods

The expression of RNAs was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effects of circ_0084615 silencing on malignant behaviors of HCC cells were assessed by CCK-8, colony formation, wound healing, and Transwell assays in vitro and tumor transplantation experiment in vivo. The expression of proteins was detected by Western blotting. Dual-luciferase reporter assay and RNA-binding protein immunoprecipitation were performed to explore the mechanism of circ_0084615.

Results

A significant upregulation of circ_0084615 was observed in HCC tissues, and positively correlated with the TNM staging. Silencing of circ_0084615 impeded HCC cell viability, colony formation, migration, invasion, epithelial-mesenchymal transition, and xenograft tumor growth. Mechanistically, circ_0084615 could bind to miR-1200 and eliminate its ability to destroy actin-like 6A (ACTL6A) mRNA, thereby increasing ACTL6A expression and facilitating the malignant behaviors of HCC cells.

Conclusions

This study clarified the oncogenic activity and mechanism of circ_0084615, thereby providing potential diagnostic biomarker and therapeutic target for inhibiting HCC progression.

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Circ_0084615可促进肝细胞癌中上皮-间质转化介导的肿瘤进展

CircRNAs 被认为是肿瘤发生和发展的关键调控因子。本研究旨在探讨 circ_0084615 在肝细胞癌（HCC）中的生物学作用和潜在机制。通过体外 CCK-8、集落形成、伤口愈合和 Transwell 试验以及体内肿瘤移植实验评估了沉默 circ_0084615 对 HCC 细胞恶性行为的影响。蛋白质的表达采用 Western 印迹法检测。在 HCC 组织中观察到 circ_0084615 的显著上调，并与 TNM 分期呈正相关。沉默circ_0084615会阻碍HCC细胞的活力、集落形成、迁移、侵袭、上皮-间质转化以及异种移植肿瘤的生长。从机制上讲，circ_0084615可与miR-1200结合，消除其破坏肌动蛋白样6A（ACTL6A）mRNA的能力，从而增加ACTL6A的表达，促进HCC细胞的恶性行为。这项研究阐明了circ_0084615的致癌活性和机制，从而为抑制HCC进展提供了潜在的诊断生物标志物和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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