DYE 10-N-NONYL ACRIDINE ORANGE AS A FLUORESCENT INDICATOR OF THE ACTION OF THE ANTISEPTIC OCTENIDINE ON THE MEMBRANES OF RHODOBACTER SPHAEROIDES CHROMATOPHORES

E. P. Lukashev, P. P. Knox, M. Strakhovskaya, V. Paschenko
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Abstract

Increasing volumes of produced antiseptics and disinfectants, which are widely used in medicine, veterinary medicine, the food industry and everyday life, can create a serious environmental problem after use. Previously, under the infl uence of micromolar concentrations of a number of antiseptics, we identifi ed disturbances in the functioning of photosynthetic membranes and phototransforming pigment-protein complexes isolated from them in various representatives of photosynthetic organisms. In this work, to determine the sensitivity of photosynthetic membranes to the action of the cationic antiseptic octenidine, we used chromatophores of the purple nonsulfur bacteria Rhodobacter sphaeroides, labeled with the fluorescent dye 10-N-nonylacridine orange (NAO). It was shown that the binding of NAO to chromatophores is accompanied by a shift in the dye emission maximum from 525 to 640 nm. The “red” fluorescence of NAO associated with chromatophores turned out to be sensitive to the effect of increasing concentrations of octenidine on photosynthetic membranes. We have found antiseptic concentrations which led to the degradation of chromatophore structures and change in the NAO aggregative state. It can be detected by an increase of “green” fluorescence in the emission spectra of the dye. The properties of NAO as a fluorescent indicator of the functional state of photosynthetic membranes and possible changes that can occur in such systems under the influence of a cationic antiseptic are discussed.
染料 10-壬基吖啶橙作为一种荧光指示剂,用于显示防腐剂辛烯胺对水合根瘤菌色素膜的作用
医药、兽医、食品工业和日常生活中广泛使用的杀菌剂和消毒剂的生产量不断增加,使用后会造成严重的环境问题。以前,在微摩尔浓度的一些防腐剂的影响下,我们发现光合膜的功能以及从光合生物的各种代表中分离出来的光转化色素-蛋白质复合物出现了紊乱。在这项工作中,为了确定光合膜对阳离子防腐剂辛烯啶作用的敏感性,我们使用了用荧光染料 10-N-壬基吖啶橙(NAO)标记的紫色非硫细菌 Rhodobacter sphaeroides 的色素层。研究表明,NAO 与色素团结合后,染料的最大发射波长从 525 纳米变为 640 纳米。事实证明,NAO 与色素团结合产生的 "红色 "荧光对光合膜上辛烯啶浓度的增加很敏感。我们发现,防腐剂的浓度可导致色团结构降解,并改变 NAO 的聚集状态。这可通过染料发射光谱中 "绿色 "荧光的增加来检测。本文讨论了NAO作为光合膜功能状态荧光指示剂的特性,以及在阳离子防腐剂的影响下,此类系统可能发生的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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