MicroRNA expression in JAG1/Notch-activated periodontal ligament stem cells.

IF 2.5 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

BDJ Open Pub Date : 2024-06-05 DOI:10.1038/s41405-024-00232-5

Promphakkon Kulthanaamondhita, Chatvadee Kornsuthisopon, Ajjima Chansaenroj, Vorapat Trachoo, Jeeranan Manokawinchoke, Lakshman Samaranayake, Supreda Suphanantachat Srithanyarat, Thanaphum Osathanon

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Abstract

Objectives: The study explored the expression profile of miRNAs in Notch-activated periodontal ligament stem cells (PDLSCs) and examined their potential cellular targets.

Methods: PDLSCs were cultured and treated with indirect immobilized Jagged1. The miRNA expression profile was examined using NanoString analysis. Bioinformatic analysis was performed together with enrichment, and miRNA expression was evaluated and validated using a quantitative polymerase chain reaction (qPCR).

Results: A total of 26 miRNAs were differentially expressed in Jagged1 treated PDLSCs compared with the controls. Pathway analysis revealed that altered miRNAs were significantly associated with the transforming growth factor β (TGF-β) signaling pathway. Target prediction analysis demonstrated that 11,170 genes as predictable targets of these altered miRNAs. Enrichment of predicted target genes revealed that they were related to ErbB, Ras and MAPK signaling pathways and small GTPase transduction.

Conclusions: The research concludes that several miRNAs are differentially expressed in jagged-1 treated PDLSCs. In translational terms the differential functionality of these miRNAs offer promise for the development of targeted regenerative materials that are necessary for managing lost tissue replacement in periodontal diseases.

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JAG1/Notch激活的牙周韧带干细胞中的微RNA表达。

研究目的本研究探讨了Notch激活的牙周韧带干细胞（PDLSCs）中miRNAs的表达谱，并研究了其潜在的细胞靶标：培养牙周韧带干细胞并用间接固定的Jagged1处理。方法：培养 PDLSCs 并用间接固定的 Jagged1 处理，使用 NanoString 分析法检测 miRNA 的表达谱。结果：共发现 26 个 miRNA：结果：与对照组相比，经 Jagged1 处理的 PDLSCs 中共有 26 个 miRNA 发生了差异表达。通路分析表明，发生变化的 miRNA 与转化生长因子 β（TGF-β）信号通路密切相关。靶标预测分析表明，11,170 个基因是这些改变的 miRNAs 的可预测靶标。预测靶基因的富集显示，它们与 ErbB、Ras 和 MAPK 信号通路以及小 GTPase 转导有关：研究得出结论：在经 jagged-1 处理的 PDLSCs 中，有几种 miRNA 的表达存在差异。从转化的角度来看，这些 miRNAs 的不同功能为开发有针对性的再生材料提供了希望，而这些材料对于管理牙周疾病中的失代偿组织是必不可少的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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