Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental Toxicology and Chemistry Pub Date : 2024-06-05 DOI:10.1002/etc.5898

William J. Martin, Paul K. Sibley, Ryan S. Prosser

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Abstract

Springtails (subclass: Collembola) represent one of the most extensively studied invertebrate groups in soil ecotoxicology. This is because of their ease of laboratory culture, significant ecological role, and sensitivity to environmental contaminants. Folsomia candida (family: Isotomidae) is a globally widespread parthenogenetic species that is prevalent in laboratory toxicity testing with springtails. Conversely, Arrhopalites caecus (family: Arrhopalitidae), a parthenogenic globular springtail species, remains untested in soil ecotoxicology. This species is found in diverse habitats, including cave systems and forest leaf litter, and has a global distribution. The sensitivity of A. caecus to environmental contaminants, such as neonicotinoid insecticides, as well as its life history and optimal culturing conditions, are largely unknown. The present study describes the establishment of a pure A. caecus laboratory culture and characterization of its life cycle and culturing conditions. We assessed the sensitivity of A. caecus to various insecticides, including exposures to the neonicotinoid thiamethoxam in soil and through a novel feeding assay as well as to clothianidin and cyantraniliprole in spiked soil exposures. In 7- and 14-day exposures to thiamethoxam in agricultural soil, the 50% lethal concentration (LC50) values were determined to be 0.129 mg/kg dry weight and 0.010 mg/kg dry weight, respectively. The 14-day LC50 for exposure to thiamethoxam via spiked food was determined to be 0.307 mg/kg dry weight. In addition, the 28-day 50% effect concentration for inhibition of juvenile production from cyantraniliprole exposure in the same soil type was 0.055 mg/kg dry weight. Challenges encountered in using this species included susceptibility to mite infestation and low adult survival rates in the 28-day cyantraniliprole test. Environ Toxicol Chem 2024;43:1820–1835. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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在土壤生态毒性测试中使用的 Springtail（Arrhopalites caecus）的特征。

鞘翅目昆虫（亚纲：鞘翅目昆虫）是土壤生态毒理学研究最为广泛的无脊椎动物类群之一。这是因为它们易于实验室培养，具有重要的生态作用，而且对环境污染物非常敏感。Folsomia candida（科：Isotomidae）是一种全球广泛分布的孤雌生殖物种，在实验室对春蜱进行毒性测试时非常普遍。相反，Arrhopalites caecus（科：Arrhopalitidae）是一种孤雌生殖的球形春尾物种，在土壤生态毒理学中仍未进行过测试。该物种栖息于多种生境，包括洞穴系统和森林落叶层，分布于全球各地。caecus 对新烟碱类杀虫剂等环境污染物的敏感性，以及它的生活史和最佳培养条件在很大程度上都是未知的。本研究介绍了建立纯净的凯氏苍蝇实验室培养物及其生命周期和培养条件的特征。我们评估了草履虫对各种杀虫剂的敏感性，包括土壤中的新烟碱噻虫嗪、新型喂食试验以及加标土壤中的噻虫嗪和氰戊菊酯。在农业土壤中接触噻虫嗪 7 天和 14 天，50%致死浓度（LC50）值分别为 0.129 毫克/千克干重和 0.010 毫克/千克干重。经测定，通过添加噻虫嗪的食物摄入噻虫嗪的 14 天半数致死浓度为 0.307 毫克/千克干重。此外，在相同的土壤类型中，暴露于氰虫酰胺 28 天抑制幼虫生产的 50%效应浓度为 0.055 毫克/千克（干重）。使用该物种遇到的挑战包括易受螨虫侵扰，以及在 28 天氰虫腈试验中成虫存活率低。环境毒物化学 2024；00：1-16。© 2024 作者。环境毒理学与化学》由 Wiley Periodicals LLC 代表 SETAC 出版。

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来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.