{"title":"Upconversion nanoparticles–based targeted imaging of MCF-7 breast cancer cells","authors":"Meric Cansu Cinar, Mahla Shahsavar Gocmen, Aysegul Aciksari, Ramazan Ceylan, Seray Sahsuvar, Sibel Cetinel, Ozgul Gok, Ayse Dulda","doi":"10.1007/s11051-024-06035-x","DOIUrl":null,"url":null,"abstract":"<p>Upconversion nanoparticles (UCNPs) doped with lanthanides are introduced as a significant tool in bioimaging applications. Here in, a comparative study has been performed to understand the cell internalization capacity of folic acid (FA) and arginine-glycine-aspartic acid-lysine (RGDK) ligands. To achieve this goal, polyacrylic acid (PAA) coated UCNPs (NaYF<sub>4</sub>:Yb<sup>3+</sup>, Er<sup>3+</sup>) are conjugated with various surface ligands such as FA and RGDK through a straightforward ligand exchange procedure. Ligand conjugation to UCNPs was characterized with a transmission electron microscope (TEM), Fourier-transform infrared (FT-IR) spectroscopy, zeta potential measurements, nuclear magnetic resonance (NMR) spectroscopy, and NanoDrop measurements. The cellular uptake of the nanoparticles was investigated on the breast cancer MCF-7 cell line. The obtained results demonstrated that folic acid and RGDK functionalized UCNPs showed remarkably higher cellular uptake, which clearly indicates that the specific targeting of UCNPs provides a better quality of sub-cellular imaging at lower energy band region.</p>","PeriodicalId":653,"journal":{"name":"Journal of Nanoparticle Research","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nanoparticle Research","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1007/s11051-024-06035-x","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Upconversion nanoparticles (UCNPs) doped with lanthanides are introduced as a significant tool in bioimaging applications. Here in, a comparative study has been performed to understand the cell internalization capacity of folic acid (FA) and arginine-glycine-aspartic acid-lysine (RGDK) ligands. To achieve this goal, polyacrylic acid (PAA) coated UCNPs (NaYF4:Yb3+, Er3+) are conjugated with various surface ligands such as FA and RGDK through a straightforward ligand exchange procedure. Ligand conjugation to UCNPs was characterized with a transmission electron microscope (TEM), Fourier-transform infrared (FT-IR) spectroscopy, zeta potential measurements, nuclear magnetic resonance (NMR) spectroscopy, and NanoDrop measurements. The cellular uptake of the nanoparticles was investigated on the breast cancer MCF-7 cell line. The obtained results demonstrated that folic acid and RGDK functionalized UCNPs showed remarkably higher cellular uptake, which clearly indicates that the specific targeting of UCNPs provides a better quality of sub-cellular imaging at lower energy band region.
期刊介绍:
The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size.
Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology.
The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.