Tailoring bisphosphonate-doped titanium films to optimally couple cellular responses and antibacterial activity for biomedical applications.

IF 1.6 4区 医学 Q4 BIOPHYSICS
Biointerphases Pub Date : 2024-05-01 DOI:10.1116/6.0003611
Leonardo F G Dias, Raphael C Costa, Catharina M Sacramento, Karina G S Ruiz, Valentim A R Barão, Paulo N Lisboa-Filho
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引用次数: 0

Abstract

Titanium (Ti) is widely utilized as an implant material; nonetheless, its integration with bone tissue faces limitations due to a patient's comorbidities. To address this challenge, we employed a strategic approach involving the growth of thin films by spin-coating and surface functionalization with etidronate (ETI), alendronate (ALE), and risedronate (RIS). Our methodology involved coating of Ti cp IV disks with thin films of TiO2, hydroxyapatite (HA), and their combinations (1:1 and 1:2 v/v), followed by surface functionalization with ETI, ALE, and RIS. Bisphosphonate-doped films were evaluated in terms of surface morphology and physical-chemical properties by techniques such as electron microscopy, confocal microscopy, and x-ray photoelectron spectroscopy. The antibacterial potential of bisphosphonates alone or functionalized onto the Ti surface was tested against Staphylococcus aureus biofilms. Primary human bone mesenchymal stem cells were used to determine in vitro cell metabolism and mineralization. Although RIS alone did not demonstrate any antibacterial effect as verified by minimum inhibitory concentration assay, when Ti surfaces were functionalized with RIS, partial inhibition of Staphylococcus aureus growth was noted, probably because of the physical-chemical surface properties. Furthermore, samples comprising TiO2/HA (1:1 and 1:2 v/v) showcased an enhancement in the metabolism of nondifferentiated cells and can potentially enhance the differentiation of osteoblastic precursors. All samples demonstrated cell viability higher than 80%. Addition of hydroxyapatite and presence of bisphosphonates increase the metabolic activity and the mineralization of human bone mesenchymal cells. While these findings hold promise, it is necessary to conduct further studies to evaluate the system's performance in vivo and ensure its long-term safety. This research marks a significant stride toward optimizing the efficacy of titanium implants through tailored surface modifications.

定制掺杂双膦酸盐的钛薄膜,使细胞反应与抗菌活性达到最佳耦合,从而实现生物医学应用。
钛(Ti)被广泛用作植入材料;然而,由于患者的合并症,钛与骨组织的结合面临着限制。为了应对这一挑战,我们采用了一种策略性方法,即通过旋涂和表面功能化的方式,用依替膦酸盐(etidronate,ETI)、阿仑膦酸盐(alendronate,ALE)和利塞膦酸盐(ridesronate,RIS)形成薄膜。我们的方法是在钛 cp IV 盘上涂覆 TiO2、羟基磷灰石(HA)和它们的组合(1:1 和 1:2 v/v)薄膜,然后用 ETI、ALE 和 RIS 进行表面功能化。通过电子显微镜、共聚焦显微镜和 X 射线光电子能谱等技术对掺杂了双膦酸盐的薄膜的表面形态和物理化学性质进行了评估。针对金黄色葡萄球菌生物膜,测试了单独或功能化在钛表面的双膦酸盐的抗菌潜力。原代人类骨间充质干细胞用于测定体外细胞代谢和矿化。通过最小抑菌浓度测定法验证,虽然单独的 RIS 没有显示出任何抗菌效果,但当用 RIS 对钛表面进行功能化时,可能由于表面的物理化学特性,部分抑制了金黄色葡萄球菌的生长。此外,TiO2/HA(1:1 和 1:2 v/v)组成的样品提高了未分化细胞的新陈代谢,并有可能促进成骨细胞前体的分化。所有样本的细胞存活率均高于 80%。添加羟基磷灰石和双膦酸盐可提高人骨间充质细胞的代谢活性和矿化度。虽然这些研究结果前景广阔,但仍有必要开展进一步研究,以评估该系统在体内的性能,并确保其长期安全性。这项研究标志着通过量身定制的表面改性在优化钛植入物功效方面迈出了重要一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biointerphases
Biointerphases 生物-材料科学:生物材料
自引率
0.00%
发文量
35
期刊介绍: Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.
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