The Contribution of Meckel's Cartilage-Derived Type II Collagen-Positive Cells to the Jawbone Development and Repair.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Hongli Chen, Yunpeng Ding, Yu Wang, Yao Sun
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引用次数: 0

Abstract

Jawbones and long bones, despite their shared skeletal lineage, frequently exhibit distinct origins and developmental pathways. Identifying specific progenitor subsets for mandibular osteogenesis remains challenging. Type II collagen is conventionally associated with cartilaginous structures, yet our investigation has identified the presence of type II collagen positive (Col2+) cells within the jawbone development and regeneration. The role of Col2+ cells in jawbone morphogenesis and repair has remained enigmatic. In this study, we analyze single-cell RNA sequencing data from mice jawbone at embryonic day 10.5. Through fate-mapping experiments, we have elucidated that Col2+ cells and their progeny are instrumental in mandibular osteogenesis across both fetal and postnatal stages. Furthermore, lineage tracing with a tamoxifen-inducible CreER system has established the pivotal role of Col2+ cells, marked by Col2-CreER and originating from the primordial Meckel's cartilage, in jawbone formation. Moreover, our research explored models simulating jawbone defects and tooth extraction, which underscored the osteogenic differentiation capabilities of postnatal Col2+ cells during repair. This finding not only highlights the regenerative potential of Col2+ cells but also suggests their versatility in contributing to skeletal healing and regeneration. In conclusion, our findings position Col2+ cells as essential in orchestrating osteogenesis throughout the continuum of mandibular development and repair.

梅克尔软骨衍生的 II 型胶原蛋白阳性细胞对颌骨发育和修复的贡献
颌骨和长骨尽管具有共同的骨骼血统,但经常表现出不同的起源和发育途径。确定下颌骨成骨的特定祖细胞亚群仍然具有挑战性。II型胶原蛋白通常与软骨结构有关,但我们的研究发现,II型胶原蛋白阳性(Col2+)细胞存在于颌骨的发育和再生过程中。Col2+ 细胞在颌骨形态发生和修复中的作用一直是个谜。在本研究中,我们分析了胚胎第 10.5 天小鼠颌骨的单细胞 RNA 测序数据。通过命运图谱实验,我们阐明了 Col2+ 细胞及其后代在胎儿期和出生后阶段的下颌骨成骨过程中的作用。此外,利用他莫昔芬诱导的CreER系统进行的系谱追踪确定了Col2+细胞在颌骨形成过程中的关键作用,这些细胞以Col2-CreER为标志,起源于原始梅克尔软骨。此外,我们的研究还探索了模拟颌骨缺损和拔牙的模型,从而强调了出生后 Col2+ 细胞在修复过程中的成骨分化能力。这一发现不仅凸显了 Col2+ 细胞的再生潜力,还表明它们在促进骨骼愈合和再生方面具有多功能性。总之,我们的研究结果表明,Col2+细胞在下颌骨发育和修复的整个过程中对协调成骨至关重要。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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