Hybrid poly(dopamine/phenosafranin) microparticles as replicas of yeast capsules for the immobilization of myoglobin

IF 4.5 3区工程技术 Q1 CHEMISTRY, APPLIED

Reactive & Functional Polymers Pub Date : 2024-05-25 DOI:10.1016/j.reactfunctpolym.2024.105946

Beata Miksa , Katarzyna Trzeciak , Marcin Górecki , Anna Kamińska , Artur Rozanski , Slawomir Kaźmierski , Mateusz Imiela , Magdalena Ziabka

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Abstract

Yeast capsules (YCs) were loaded with a photosensitive phenosafranin (PSF) dye through electrostatic force-driven spontaneous deposition and served as templates for synthesizing biocompatible microparticles. Oppositely charged dopamine and PSF were encapsulated and deposited onto the yeast polysaccharide scaffold through ionic complexation. Subsequently, self-oxidative polymerization was carried out in situ, forming YC@(PDPSF) microparticles. To extend an innovative idea for diverse design and multipurpose YC@(PDPSF) applications, we immobilized myoglobin (Mb) on their surface. The multifunctional coating facilitated the immobilization of Mb involved in oxygen (O₂) storage utilizing the carbodiimide method with 6-aminocaproic acid (ACA) as a linker. The biological activity of Mb encapsulated in YCs and immobilized on the surface of YC@(PDPSF) microparticles was compared using UV–vis spectrophotometry. The assays showed that the catalytic activity of Mb was sufficiently retained after immobilization. This new YC@(PDPSF)-Mb microparticles will have potential applications in biosensors, particularly for detecting and separating specific analytes and small molecules (e.g. NO, CO). Their unique properties could make them valuable for various biomedical purposes including enzyme assays, chromatography, air pollution control devices, and filtration processes in gas mask filters.

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作为固定肌红蛋白的酵母胶囊复制品的杂化聚（多巴胺/酚afranin）微颗粒

通过静电力驱动的自发沉积作用，酵母胶囊（YCs）负载了一种光敏苯并afranin（PSF）染料，并以此为模板合成了生物相容性微粒。带相反电荷的多巴胺和 PSF 通过离子络合被包裹并沉积在酵母多糖支架上。随后，在原位进行自氧化聚合，形成 YC@（PDPSF）微颗粒。为了拓展 YC@(PDPSF)的多样化设计和多用途应用，我们在其表面固定了肌红蛋白（Mb）。利用碳二亚胺法，以 6-氨基己酸（ACA）为连接剂，多功能涂层有助于固定参与氧气（O2）储存的 Mb。利用紫外可见分光光度法比较了封装在 YCs 中的 Mb 和固定在 YC@（PDPSF）微颗粒表面的 Mb 的生物活性。检测结果表明，Mb 的催化活性在固定后得到了充分的保留。这种新型 YC@(PDPSF)-Mb 微颗粒将有可能应用于生物传感器，特别是用于检测和分离特定的分析物和小分子（如 NO、CO）。这些微粒的独特性质可使其在各种生物医学用途中发挥重要作用，包括酶测定、色谱法、空气污染控制装置以及防毒面具过滤器中的过滤过程。

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来源期刊

Reactive & Functional Polymers 工程技术-高分子科学

CiteScore

8.90

自引率

5.90%

发文量

259

审稿时长

27 days

期刊介绍： Reactive & Functional Polymers provides a forum to disseminate original ideas, concepts and developments in the science and technology of polymers with functional groups, which impart specific chemical reactivity or physical, chemical, structural, biological, and pharmacological functionality. The scope covers organic polymers, acting for instance as reagents, catalysts, templates, ion-exchangers, selective sorbents, chelating or antimicrobial agents, drug carriers, sensors, membranes, and hydrogels. This also includes reactive cross-linkable prepolymers and high-performance thermosetting polymers, natural or degradable polymers, conducting polymers, and porous polymers. Original research articles must contain thorough molecular and material characterization data on synthesis of the above polymers in combination with their applications. Applications include but are not limited to catalysis, water or effluent treatment, separations and recovery, electronics and information storage, energy conversion, encapsulation, or adhesion.