Dietary α-linolenic acid supplementation enhances resistance to Salmonella Typhimurium challenge in chickens by altering the intestinal mucosal barrier integrity and cecal microbes

IF 6.1 1区生物学 Q1 MICROBIOLOGY

Microbiological research Pub Date : 2024-05-31 DOI:10.1016/j.micres.2024.127773

Boheng Ma , De Wang , Xuan Chen , Qin Wang , Tiejun Zhang , Renqiao Wen , Ming Yang , Cui Li , Changwei Lei , Hongning Wang

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引用次数: 0

Abstract

Salmonella is an important foodborne pathogen. Given the ban on the use of antibiotics during the egg-laying period in China, finding safe and effective alternatives to antibiotics to reduce Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) infections in chickens is essential for the prevention and control of this pathogen and the protection of human health. Numerous studies have shown that unsaturated fatty acids have a positive effect on intestinal inflammation and resistance to infection by intestinal pathogens. Here we investigated the protective effect of α-linolenic acid (ALA) against S. Typhimurium infection in chickens and further explored its mechanism of action. We added different proportions of ALA to the feed and observed the effect of ALA on S. Typhimurium colonization using metagenomic sequencing technology and physiological index measurements. The role of gut flora on S. Typhimurium colonization was subsequently verified by fecal microbiota transplantation (FMT). We found that ALA protects chickens from S. Typhimurium infection by reducing intestinal inflammation through remodeling the gut microbiota, up-regulating the expression of ileocecal barrier-related genes, and maintaining the integrity of the intestinal epithelium. Our data suggest that supplementation of feed with ALA may be an effective strategy to alleviate S. Typhimurium infection in chickens.

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通过改变肠粘膜屏障完整性和盲肠微生物补充α-亚麻酸可增强鸡对鼠伤寒沙门氏菌挑战的抵抗力

沙门氏菌是一种重要的食源性病原体。鉴于中国禁止在蛋鸡产蛋期使用抗生素，因此寻找安全有效的抗生素替代品来减少鸡肠炎沙门氏菌（S. Typhimurium）感染，对于预防和控制这种病原体以及保护人类健康至关重要。大量研究表明，不饱和脂肪酸对肠道炎症和抵抗肠道病原体感染有积极作用。在此，我们研究了α-亚麻酸（ALA）对鸡感染伤寒杆菌的保护作用，并进一步探讨了其作用机制。我们在饲料中添加了不同比例的 ALA，并利用元基因组测序技术和生理指标测量方法观察了 ALA 对伤寒杆菌定植的影响。随后通过粪便微生物群移植（FMT）验证了肠道菌群对伤寒杆菌定植的作用。我们发现，ALA 可通过重塑肠道微生物群、上调回盲屏障相关基因的表达以及维持肠道上皮的完整性来减少肠道炎症，从而保护鸡免受鼠伤寒杆菌感染。我们的数据表明，在饲料中补充 ALA 可能是缓解鸡伤寒杆菌感染的有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiological research 生物-微生物学

CiteScore

10.90

自引率

6.00%

发文量

249

审稿时长

29 days

期刊介绍： Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.