Exosomal microRNAs associated with tuberculosis among people living with human immunodeficiency virus

IF 1.9 Q3 INFECTIOUS DISEASES

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Pub Date : 2024-05-31 DOI:10.1016/j.jctube.2024.100453

Yujiao Jin, Yuan Liu, Wenyan Yu, Yan Zhang, Kenv Pan, Miaochan Wang, Aifang Xu

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引用次数: 0

Abstract

Objective

To investigate the diagnostic value of selected exosomal miRNAs for Tuberculosis (TB) among people living with human immunodeficiency virus (PLHIV).

Methods

A total of 43 adult HIV patients, including 20 diagnosed with TB and 23 controls, were enrolled. The levels of six exosomal miRNAs (miR-20a, miR-20b, miR-26a, miR-106a, miR-191, and miR-486) were measured using qRT-PCR.

Results

The levels of these six exosomal miRNAs (miR-20a, miR-20b, miR-26a, miR-106a, miR-191, and miR-486) were significantly higher in the plasma of TB patients compared to controls among PLHIV. The Receiver Operating Characteristic (ROC) curve of these six miRNAs showed a fair performance in distinguishing TB patients from controls, with Area Under Curve (AUC) values of 0.78 (95 %CI 0.63–0.93), 0.81 (95 %CI 0.67–0.95), 0.77 (95 %CI 0.61–0.93), 0.84 (95 %CI 0.70–0.98), 0.82 (95 %CI 0.68–0.95) and 0.79 (95 %CI 0.65–0.93), respectively. These miRNAs showed higher AUC values for extrapulmonary tuberculosis compared to pulmonary tuberculosis. An analysis of subgroups was performed based on CD4 + T cell count (＜200 and ≥ 200 cells·µL⁻¹). In the high CD4 count group, all these six exosomal miRNAs appeared to have higher AUC values compared to the low CD4 count group.

Conclusions

These six exosomal miRNAs could serve as potential biomarkers for diagnosing TB among PLHIV.

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与人类免疫缺陷病毒感染者结核病有关的外泌体微RNA

方法共招募了 43 名成年艾滋病病毒感染者，包括 20 名确诊为肺结核的患者和 23 名对照组患者。结果与对照组相比，肺结核患者血浆中六种外泌体 miRNA（miR-20a、miR-20b、miR-26a、miR-106a、miR-191 和 miR-486）的水平明显较高。这六种 miRNA 的接收者操作特征曲线（ROC）显示，它们在区分结核病患者和对照组方面表现尚可，曲线下面积（AUC）值为 0.78（95 %CI 0.63-0.93）、0.81（95 %CI 0.67-0.95）、0.77（95 %CI 0.61-0.93）、0.84（95 %CI 0.70-0.98）、0.82（95 %CI 0.68-0.95）和 0.79（95 %CI 0.65-0.93）。与肺结核相比，肺外结核的这些 miRNA 的 AUC 值更高。根据 CD4 + T 细胞计数（＜200 和≥200 cells-µL-1）对亚组进行了分析。结论这六种外泌体 miRNA 可作为诊断 PLHIV 中结核病的潜在生物标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Medicine-Pulmonary and Respiratory Medicine

CiteScore

4.00

自引率

5.00%

发文量

审稿时长

30 weeks

期刊介绍： Journal of Clinical Tuberculosis and Mycobacterial Diseases aims to provide a forum for clinically relevant articles on all aspects of tuberculosis and other mycobacterial infections, including (but not limited to) epidemiology, clinical investigation, transmission, diagnosis, treatment, drug-resistance and public policy, and encourages the submission of clinical studies, thematic reviews and case reports. Journal of Clinical Tuberculosis and Mycobacterial Diseases is an Open Access publication.