Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Adhesion & Migration Pub Date : 2024-12-01 Epub Date: 2024-06-03 DOI:10.1080/19336918.2024.2353920
Jia Yang, Xiaoni Zhong, Xiaoling Gao, Wenyi Xie, Yaokai Chen, Yuanjiang Liao, Peilin Zhang
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引用次数: 0

Abstract

In this research, we investigated the role of PIK3R6, a regulatory subunit of PI3Kγ, known for its tumor-promoting properties, in clear cell renal cell carcinoma (CCRCC). Utilizing the UALCAN website, we found PIK3R6 upregulated in CCRCC, correlating with lower survival rates. We compared PIK3R6 expression in CCRCC tumor tissues and adjacent normal tissues using immunohistochemistry. Post RNA interference-induced knockdown of PIK3R6 in 786-O and ACHN cell lines, we performed CCK-8, colony formation, Edu staining, flow cytometry, wound healing, and transwell assays. Results showed that PIK3R6 silencing reduced cell proliferation, migration, and invasion, and induced G0/G1 phase arrest and apoptosis. Molecular analysis revealed decreased CDK4, Cyclin D1, N-cadherin, Vimentin, Bcl-2, p-PI3K and p-AKT, with increased cleaved caspase-3, Bax, and E-cadherin levels in CCRCC cells. Moreover, inhibiting PIK3R6 hindered tumor growth. These findings suggest a significant role for PIK3R6 in CCRCC cell proliferation and metastasis, presenting it as a potential therapeutic target.

敲除 PIK3R6 会阻碍透明细胞肾细胞癌的发生和发展。
在这项研究中,我们调查了PIK3R6在透明细胞肾细胞癌(CCRCC)中的作用,PIK3R6是PI3Kγ的一个调节亚基,以其肿瘤促进特性而闻名。利用 UALCAN 网站,我们发现 PIK3R6 在 CCRCC 中上调,与较低的生存率相关。我们使用免疫组化方法比较了 PIK3R6 在 CCRCC 肿瘤组织和邻近正常组织中的表达。在 RNA 干扰诱导敲除 786-O 和 ACHN 细胞系中的 PIK3R6 后,我们进行了 CCK-8、集落形成、Edu 染色、流式细胞术、伤口愈合和透孔试验。结果表明,PIK3R6沉默可减少细胞增殖、迁移和侵袭,并诱导细胞G0/G1期停滞和凋亡。分子分析表明,CCRCC细胞中CDK4、细胞周期蛋白D1、N-钙粘蛋白、波形蛋白、Bcl-2、p-PI3K和p-AKT水平降低,裂解的Caspase-3、Bax和E-钙粘蛋白水平升高。此外,抑制 PIK3R6 会阻碍肿瘤生长。这些研究结果表明,PIK3R6 在 CCRCC 细胞增殖和转移过程中发挥着重要作用,是一个潜在的治疗靶点。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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