Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-06-21 DOI:10.1093/bbb/zbae061

Taiki Kuzuhara, Kota Monden, Takushi Hachiya, Tsuyoshi Nakagawa

引用次数: 0

Abstract

Gateway cloning is a useful technology for the simple and reliable preparation of various construct in many organisms. However, there is a problem regarding the negative control construct in the Gateway cloning system. In this study, we developed the pENTR-NeCo-lacZα vector system to create an empty vector that can be used as a negative control construct in Gateway cloning.

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开发 pENTR-NeCo-lacZα 载体，用于在 Gateway 克隆中制备阴性对照构建体。

Gateway克隆是一种有用的技术，可以简单可靠地在许多生物体内制备各种构建体。然而，Gateway 克隆系统中存在一个关于阴性对照构建体的问题。在本研究中，我们开发了 pENTR-NeCo-lacZα 载体系统，以创建一种空载体，可用作 Gateway 克隆中的阴性对照构建体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).