Bioactive glass 1393 promotes angiogenesis and accelerates wound healing through ROS/P53/MMP9 signaling pathway

IF 3.4 3区环境科学与生态学 Q3 CELL & TISSUE ENGINEERING

Regenerative Therapy Pub Date : 2024-06-01 DOI:10.1016/j.reth.2024.05.016

Xuenan Chen , Xinyu Ran , Xuebo Wei , Lifei Zhu , Shaodong Chen , Zhiyong Liao , Ke Xu , Weidong Xia

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引用次数: 0

Abstract

Compared to bioactive glass 45S5, bioactive glass 1393 has shown greater potential in activating tissue cells and promoting angiogenesis for bone repair. Nevertheless, the effect of bioactive glass 1393 in the context of wound healing remains extensively unexplored, and its mechanism in wound healing remains unclear. Considering that angiogenesis is a critical stage in wound healing, we hypothesize that bioactive glass 1393 may facilitate wound healing through the stimulation of angiogenesis. To validate this hypothesis and further explore the mechanisms underlying its pro-angiogenic effects, we investigated the impact of bioactive glass 1393 on wound healing angiogenesis through both in vivo and in vitro studies. The research demonstrated that bioactive glass 1393 accelerated wound healing by promoting the formation of granulation, deposition of collagen, and angiogenesis. The results of Western blot analysis and immunofluorescence staining revealed that bioactive glass 1393 up-regulated the expression of angiogenesis-related factors. Additionally, bioactive glass 1393 inhibited the expression of ROS and P53 to promote angiogenesis. Furthermore, bioactive glass 1393 stimulated angiogenesis through the P53 signaling pathway, as evidenced by P53 activation assays. Collectively, these findings indicate that bioactive glass 1393 accelerates wound healing by promoting angiogenesis via the ROS/P53/MMP9 signaling pathway.

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生物活性玻璃 1393 通过 ROS/P53/MMP9 信号通路促进血管生成并加速伤口愈合

与生物活性玻璃 45S5 相比，生物活性玻璃 1393 在激活组织细胞和促进血管生成以修复骨质方面具有更大的潜力。然而，生物活性玻璃 1393 在伤口愈合方面的作用仍未得到广泛探索，其在伤口愈合中的作用机制也仍不清楚。考虑到血管生成是伤口愈合的关键阶段，我们假设生物活性玻璃 1393 可通过刺激血管生成促进伤口愈合。为了验证这一假设并进一步探索其促进血管生成作用的机制，我们通过体内和体外研究，探讨了生物活性玻璃 1393 对伤口愈合血管生成的影响。研究表明，生物活性玻璃 1393 能促进肉芽形成、胶原沉积和血管生成，从而加速伤口愈合。Western 印迹分析和免疫荧光染色结果显示，生物活性玻璃 1393 能上调血管生成相关因子的表达。此外，生物活性玻璃 1393 还能抑制 ROS 和 P53 的表达，促进血管生成。此外，生物活性玻璃 1393 还能通过 P53 信号通路刺激血管生成，这一点已在 P53 激活试验中得到证实。总之，这些研究结果表明，生物活性玻璃 1393 可通过 ROS/P53/MMP9 信号通路促进血管生成，从而加速伤口愈合。

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来源期刊

Regenerative Therapy Engineering-Biomedical Engineering

CiteScore

6.00

自引率

2.30%

发文量

106

审稿时长

49 days

期刊介绍： Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.