Physicochemical properties of Eri pupae flakes and their antibacterial activity against Salmonella sp., Escherichia coli and Staphylococcus aureus

IF 0.4 Q4 MICROBIOLOGY
Y. Endrawati, A. Apriantini, Noviyan Darmawan, Rini Madyastuti Purwono
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引用次数: 0

Abstract

Aims: The objective of this study was to evaluate the physicochemical properties of Eri silkworm ( Samia cynthia ricini ) pupae flour, specifically the pH value, water activity, water content, fat value, ash value and protein value. The study was also aimed to determine the antibacterial activity of Eri silkworm pupae flour against Salmonella sp., Escherichia coli and Staphylococcus aureus . Methodology and results: Pupae meal is a flour made from the extract of the Eri silkworm pupae used as a flour substitute in producing pupae flakes. Pupae meal had two particle size treatments, 60 and 80 mesh, whereas pupae flake had three pupae meal substitution treatments of 0%, 4% and 8%. The physicochemical properties, antioxidant activity and microbiology of pupae meal and flake were analyzed. The results showed that Eri pupae flour met the Indonesia National Standard for Cereals (SNI 01-4270-1996) requirements for food ingredients regarding pH value, water activity, water content, fat value and protein value. The flour also exhibited antibacterial activity against all three bacterial species tested, with S. aureus being the most susceptible. Antioxidant activity and capacity increased as the percentage of pupae meal in the pupae flakes increased. The IC 50 value of pupae meal, 68 µg/mL, was included in the strong category. Salmonella sp. were not found in the pupae meal and flake. Total plate count, E. coli and S. aureus numbers remained within food tolerance limits based on the Indonesia National Standard in both pupae flour and pupae flakes. Conclusion, significance and impact of study: The particle size of pupae flour did not affect its physicochemical properties or antioxidant activity, and microbiologically, it met the Indonesia National Standard for flour. Moreover, The flakes product with pupae flour substitution influenced its physicochemical properties, with the best level at 8% pupae flour for the highest protein content and increased the flakes product's activity and antioxidant capacity. Based on the microbiological analysis of pupa flakes products, The microbial content in the pupa was still within safe limits for human consumption. Research findings suggest that pupae flakes are in accordance with the Indonesia National Standard for Cereal and are a good alternative as a functional food.
埃里蚕蛹片的理化特性及其对沙门氏菌、大肠杆菌和金黄色葡萄球菌的抗菌活性
目的:本研究的目的是评估埃里蚕蛹粉的理化性质,特别是 pH 值、水活性、含水量、脂肪值、灰分值和蛋白质值。研究还旨在确定蚕蛹粉对沙门氏菌、大肠杆菌和金黄色葡萄球菌的抗菌活性。方法和结果蚕蛹粉是一种用蚕蛹提取物制成的面粉,在生产蚕蛹片时用作面粉替代品。蚕蛹粉有两种粒度处理,分别为 60 目和 80 目,而蚕蛹片有三种蚕蛹粉替代物处理,分别为 0%、4% 和 8%。对蚕蛹粉和蚕蛹片的理化特性、抗氧化活性和微生物学进行了分析。结果表明,埃里蚕蛹粉符合印度尼西亚国家谷物标准(SNI 01-4270-1996)对食品配料的 pH 值、水活性、含水量、脂肪值和蛋白质值的要求。该面粉还对所有三种受测细菌具有抗菌活性,其中金黄色葡萄球菌最易感。抗氧化活性和能力随着蚕蛹片中蚕蛹粉比例的增加而提高。蚕蛹粉的 IC 50 值为 68 µg/mL,属于强抗氧化剂。在蚕蛹粉和蚕蛹片中没有发现沙门氏菌。根据印度尼西亚国家标准,蚕蛹粉和蚕蛹片中的菌落总数、大肠杆菌和金黄色葡萄球菌数量均未超过食品耐受限度。研究的结论、意义和影响:蚕蛹粉的粒度不会影响其理化特性或抗氧化活性,在微生物方面也符合印尼面粉国家标准。此外,蚕蛹粉替代物对薄片产品的理化特性有影响,蛋白质含量最高的蚕蛹粉含量最好为 8%,并且提高了薄片产品的活性和抗氧化能力。根据对蚕蛹片产品的微生物分析,蚕蛹中的微生物含量仍在人类食用的安全范围内。研究结果表明,蚕蛹片符合印尼国家谷物标准,是功能性食品的良好替代品。
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来源期刊
CiteScore
0.70
自引率
0.00%
发文量
43
审稿时长
11 weeks
期刊介绍: The Malaysian Journal of Microbiology (MJM) publishes high quality microbiology research related to the tropics. These include infectious diseases and antimicrobials. In addition, the journal also publishes research works on the application of microbes for the betterment of human society and the environment. The journal welcomes papers on isolation, identification, characterization and application of microbes and microbial products. The MJM is published under the auspices of the Malaysian Society for Microbiology. It serves as a forum for scientific communication among scientists and academics who deal with microbes and microbial products. The journal publishes research articles, short communications and review articles on various novel aspects of microbiology, which include topics related to medical, pharmaceutical, food, agricultural, industry, plant pathology, biotechnology, microbial genetics, environment, soil, water and biodeterioration. The journal aspires to emphasize the important roles played by microbes in our daily life.
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