Characterizing intracellular mechanics via optical tweezers-based microrheology

IF 6 2区 生物学 Q1 CELL BIOLOGY
Bart E. Vos , Till M. Muenker , Timo Betz
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引用次数: 0

Abstract

Intracellular organization is a highly regulated homeostatic state maintained to ensure eukaryotic cells’ correct and efficient functioning. Thanks to decades of research, vast knowledge of the proteins involved in intracellular transport and organization has been acquired. However, how these influence and potentially regulate the intracellular mechanical properties of the cell is largely unknown. There is a deep knowledge gap between the understanding of cortical mechanics, which is accessible by a series of experimental tools, and the intracellular situation that has been largely neglected due to the difficulty of performing intracellular mechanics measurements. Recently, tools required for such quantitative and localized analysis of intracellular mechanics have been introduced. Here, we review how these approaches and the resulting viscoelastic models lead the way to a full mechanical description of the cytoplasm, which is instrumental for a quantitative characterization of the intracellular life of cells.

通过基于光学镊子的微流变学表征细胞内力学。
细胞内的组织是一种高度调节的平衡状态,以确保真核细胞的正确和高效运作。经过数十年的研究,人们对参与细胞内运输和组织的蛋白质有了大量了解。然而,这些蛋白质如何影响并潜在地调节细胞内的机械特性,在很大程度上还是未知数。由于难以进行细胞内力学测量,人们对皮层力学的了解与细胞内情况之间存在着深刻的知识鸿沟。最近,对细胞内力学进行定量和定位分析所需的工具已经问世。在此,我们将回顾这些方法和由此产生的粘弹性模型是如何引导对细胞质进行全面力学描述的,这对细胞内生命的定量表征非常重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Opinion in Cell Biology
Current Opinion in Cell Biology 生物-细胞生物学
CiteScore
14.60
自引率
1.30%
发文量
79
审稿时长
93 days
期刊介绍: Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.
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