High-strength and high-elasticity silk fibroin-composite gelatin biomaterial hydrogels for rabbit knee cartilage regeneration

IF 2.6 4区 材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY
Hebin Ma, Bowen Xie, Hongguang Chen, Puzhen Song, Yuanbo Zhou, Haigang Jia, Jing Liu, Yantao Zhao, Yadong Zhang
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引用次数: 0

Abstract

Suitable hydrogel materials for cartilage tissue repair should exhibit high strength and toughness, and excellent biocompatibility. However, the mechanical properties of most hydrogels cannot meet the complex mechanical requirements of articular cartilage tissues. Given this situation, we have adopted a chemical cross-linking method using hexafluoro isopropanol to mediate the cross-linking of Silk Fibroin (SF) and deionized water (DI), which promoted the formation of β-sheets, generating “high-toughness” Silk Fibroin hydrogels. The introduction of Gelatin (Gel) served to increase the content of β-sheets and increase the tensile modulus from 24.51 ± 2.07 MPa to 39.75 ± 6.54 MPa, which significantly enhanced the flexibility of the hydrogel and meets the mechanical requirements of cartilage tissue. In addition, in vitro biological experiments have shown that the introduction of Gel promotes cell proliferation and enhances the production of cartilage extracellular matrix by chondrocytes. In vivo experiments have demonstrated that SF/Gel hydrogel promotes articular cartilage regeneration more effectively than SF hydrogel, as evidenced by improvements in gross appearance, imaging, and histology. This study has established that high-strength SF/Gel hydrogel prepared by applying the binary-solvent-induced conformation transition strategy has potential applications in cartilage tissue repair and regeneration and is a feasible biomaterial for osteochondral regeneration.
用于兔膝关节软骨再生的高强度和高弹性丝纤维蛋白复合明胶生物材料水凝胶
用于软骨组织修复的合适水凝胶材料应具有高强度和韧性,以及良好的生物相容性。然而,大多数水凝胶的机械性能无法满足关节软骨组织复杂的机械要求。鉴于这种情况,我们采用了一种化学交联方法,利用六氟异丙醇介导蚕丝纤维素(SF)与去离子水(DI)交联,促进了β-片层的形成,生成了 "高韧性 "蚕丝纤维素水凝胶。明胶(Gel)的引入增加了β-片的含量,使拉伸模量从 24.51 ± 2.07 兆帕增加到 39.75 ± 6.54 兆帕,从而显著提高了水凝胶的柔韧性,满足了软骨组织的力学要求。此外,体外生物实验表明,Gel 的引入能促进细胞增殖,增强软骨细胞产生软骨细胞外基质。体内实验表明,与 SF 水凝胶相比,SF/Gel 水凝胶能更有效地促进关节软骨的再生,这体现在大体外观、成像和组织学方面。这项研究证实,采用二元溶剂诱导构象转变策略制备的高强度 SF/Gel 水凝胶在软骨组织修复和再生方面具有潜在的应用价值,是一种可行的骨软骨再生生物材料。
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来源期刊
Frontiers in Materials
Frontiers in Materials Materials Science-Materials Science (miscellaneous)
CiteScore
4.80
自引率
6.20%
发文量
749
审稿时长
12 weeks
期刊介绍: Frontiers in Materials is a high visibility journal publishing rigorously peer-reviewed research across the entire breadth of materials science and engineering. This interdisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers across academia and industry, and the public worldwide. Founded upon a research community driven approach, this Journal provides a balanced and comprehensive offering of Specialty Sections, each of which has a dedicated Editorial Board of leading experts in the respective field.
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