Species-level understanding of the bacterial community in Daqu based on full-length 16S rRNA gene sequences

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Pei-Jie Han , Liang Song , Zhang Wen , Hai-Yan Zhu , Yu-Hua Wei , Jian-Wei Wang , Mei Bai , Lu-Jun Luo , Ju-Wei Wang , Shen-Xi Chen , Xiao-Long You , Da-Yong Han , Feng-Yan Bai
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引用次数: 0

Abstract

Daqu is used as the fermentation starter of Baijiu and contributes diversified functional microbes for saccharifying grains and converting sugars into ethanol and aroma components in Baijiu products. Daqu is mainly classified into three types, namely low (LTD), medium (MTD) and high (HTD) temperature Daqu, according to the highest temperatures reached in their fermentation processes. In this study, we used the PacBio small-molecule real-time (SMRT) sequencing technology to determine the full-length 16 S rRNA gene sequences from the metagenomes of 296 samples of different types of Daqu collected from ten provinces in China, and revealed the bacterial diversity at the species level in the Daqu samples. We totally identified 310 bacteria species, including 78 highly abundant species (with a relative abundance >0.1% each) which accounted for 91.90% of the reads from all the Daqu samples. We also recognized the differentially enriched bacterial species in different types of Daqu, and in the Daqu samples with the same type but from different provinces. Specifically, Lactobacillales, Enterobacterales and Bacillaceae were significantly enriched in the LTD, MTD and HTD groups, respectively. The potential co-existence and exclusion relationships among the bacteria species involved in all the Daqu samples and in the LTD, MTD and HTD samples from a specific region were also identified. These results provide a better understanding of the bacterial diversity in different types of Daqu at the species level.

Abstract Image

Abstract Image

基于全长 16S rRNA 基因序列从物种层面了解大曲细菌群落
大曲作为白酒的发酵启动剂,为白酒产品中的谷物糖化和糖转化为乙醇和香味成分提供了多样化的功能微生物。大曲根据其发酵过程中达到的最高温度,主要分为低温大曲(LTD)、中温大曲(MTD)和高温大曲(HTD)三种类型。本研究采用 PacBio 小分子实时(SMRT)测序技术,测定了从中国十个省份采集的 296 份不同类型大曲样品元基因组的全长 16 S rRNA 基因序列,揭示了大曲样品中物种水平的细菌多样性。我们共鉴定出 310 个细菌物种,其中 78 个高丰度物种(每个物种的相对丰度大于 0.1%)占所有大曲样品读数的 91.90%。我们还识别了不同类型大曲中的不同富集菌种,以及同一类型但来自不同省份的大曲样品中的不同富集菌种。具体而言,乳杆菌属、肠杆菌属和芽孢杆菌属分别在LTD组、MTD组和HTD组中显著富集。此外,还确定了所有大曲样品和来自特定地区的大曲、中曲和高曲样品中涉及的细菌种类之间可能存在的共存和排斥关系。这些结果有助于从物种层面更好地了解不同类型大曲中的细菌多样性。
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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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