LncRNA NEAT1/miR-211/IL-10 Axis Regulates Inflammation of Peripheral Blood Mononuclear Cells in Acute Myocardial Infarction

IF 1.2 4区 医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS
Min-na Hou, Gang-jun Zong, Ying Sun, Jia-jia Jiang, Jun Ding
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Abstract

This study aimed to explore the expression of long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) in patients with acute myocardial infarction (AMI) and its inflammatory regulation mechanism through miR-211/interleukin 10 (IL-10) axis.

A total of 75 participants were enrolled in this study: 25 healthy people in the control group, 25 patients with stable angina pectoris (SAP) in the SAP group, and 25 patients with AMI in the AMI group. Real-time qPCR was used to detect mRNA expression levels of NEAT1, miR-211, and IL-10. The interaction between miR-211, NEAT1, and IL-10 was confirmed by dual-luciferase reporter assay, and protein expression was detected using western blot.

High expression of NEAT1 in peripheral blood mononuclear cells (PBMCs) of patients with AMI was negatively related to serum creatine kinase-MB (CK-MB), cardiac troponin I (cTnI), tumor necrosis factor-α (TNF-α), IL-6, and IL-1β and was positively correlated with left ventricular ejection fraction (LVEF). In THP-1 cells, miR-211 was confirmed to target and inhibit IL-10 expression. NEAT1 knockdown and miR-211-mimic markedly decreased IL-10 protein levels, whereas anti-miR-211 markedly increased IL-10 protein levels. Importantly, miR-211 level was negatively related to NEAT1 and IL-10 levels, whereas IL-10 level was positively related to the level of NEAT1 expression in PBMCs of patients with AMI.

LncRNA NEAT1 was highly expressed in PBMCs of patients with AMI, and NEAT1 suppressed inflammation via miR-211/IL-10 axis in PBMCs of patients with AMI.

LncRNA NEAT1/miR-211/IL-10 轴调控急性心肌梗死时外周血单核细胞的炎症反应
本研究旨在探讨长非编码RNA(lncRNA)核旁组装转录本1(NEAT1)在急性心肌梗死(AMI)患者中的表达及其通过miR-211/白细胞介素10(IL-10)轴的炎症调控机制。本研究共纳入75名参与者:25名健康人为对照组,25名稳定型心绞痛(SAP)患者为SAP组,25名AMI患者为AMI组。研究采用实时 qPCR 技术检测 NEAT1、miR-211 和 IL-10 的 mRNA 表达水平。通过双荧光素酶报告实验证实了 miR-211、NEAT1 和 IL-10 之间的相互作用,并通过 Western 印迹检测了蛋白表达。AMI 患者外周血单核细胞(PBMC)中 NEAT1 的高表达与血清肌酸激酶-MB(CK-MB)、心肌肌钙蛋白 I(cTnI)、肿瘤坏死因子-α(TNF-α)、IL-6 和 IL-1β 呈负相关,与左心室射血分数(LVEF)呈正相关。在 THP-1 细胞中,miR-211 被证实靶向并抑制 IL-10 的表达。NEAT1敲除和miR-211模拟物明显降低了IL-10蛋白水平,而抗miR-211则明显提高了IL-10蛋白水平。重要的是,在 AMI 患者的 PBMCs 中,miR-211 水平与 NEAT1 和 IL-10 水平呈负相关,而 IL-10 水平与 NEAT1 表达水平呈正相关。
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来源期刊
International heart journal
International heart journal 医学-心血管系统
CiteScore
2.50
自引率
6.70%
发文量
148
审稿时长
6-12 weeks
期刊介绍: Authors of research articles should disclose at the time of submission any financial arrangement they may have with a company whose product figures prominently in the submitted manuscript or with a company making a competing product. Such information will be held in confidence while the paper is under review and will not influence the editorial decision, but if the article is accepted for publication, the editors will usually discuss with the authors the manner in which such information is to be communicated to the reader.
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