LncRNA Peg13 Alleviates Myocardial Infarction/Reperfusion Injury through Regulating MiR-34a/Sirt1-Mediated Endoplasmic Reticulum Stress

IF 1.2 4区 医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS
Yonghong Wang, Jian Luo, Huiqiong Yang, Yanfei Liu
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Abstract

Myocardial infarction/reperfusion (I/R) injury significantly impacts the health of older individuals. We confirmed that the level of lncRNA Peg13 was downregulated in I/R injury. However, the detailed function of Peg13 in myocardial I/R injury has not yet been explored.

To detect the function of Peg13, in vivo model of I/R injury was constructed. RT-qPCR was employed to investigate RNA levels, and Western blotting was performed to assess levels of endoplasmic reticulum stress and apoptosis-associated proteins. EdU staining was confirmed to assess the cell proliferation.

I/R therapy dramatically produced myocardial injury, increased the infarct area, and decreased the amount of Peg13 in myocardial tissues of mice. In addition, hypoxia/reoxygenation (H/R) notably induced the apoptosis and promoted the endoplasmic reticulum (ER) stress of HL-1 cells, while overexpression of Peg13 reversed these phenomena. Additionally, Peg13 may increase the level of Sirt1 through binding to miR-34a. Upregulation of Peg13 reversed H/R-induced ER stress via regulation of miR-34a/Sirt1 axis.

LncRNA Peg13 reduces ER stress in myocardial infarction/reperfusion injury through mediation of miR-34a/Sirt1 axis. Hence, our research might shed new lights on developing new strategies for the treatment of myocardial I/R injury.

LncRNA Peg13 通过调节 MiR-34a/Sirt1 介导的内质网应激减轻心肌梗死/再灌注损伤
心肌梗死/再灌注(I/R)损伤严重影响老年人的健康。我们证实,lncRNA Peg13的水平在I/R损伤中下调。为了检测Peg13的功能,我们构建了I/R损伤的体内模型。为了检测Peg13的功能,研究人员构建了I/R损伤体内模型,采用RT-qPCR检测RNA水平,并进行Western印迹以评估内质网应激和细胞凋亡相关蛋白的水平。I/R疗法会显著造成心肌损伤,增加梗死面积,减少小鼠心肌组织中Peg13的含量。此外,缺氧/复氧(H/R)显著诱导 HL-1 细胞凋亡并促进内质网(ER)应激,而过表达 Peg13 则可逆转这些现象。此外,Peg13 还可能通过与 miR-34a 结合提高 Sirt1 的水平。LncRNA Peg13通过调控miR-34a/Sirt1轴降低心肌梗死/再灌注损伤中的ER应激。因此,我们的研究可能为开发治疗心肌I/R损伤的新策略提供新的启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International heart journal
International heart journal 医学-心血管系统
CiteScore
2.50
自引率
6.70%
发文量
148
审稿时长
6-12 weeks
期刊介绍: Authors of research articles should disclose at the time of submission any financial arrangement they may have with a company whose product figures prominently in the submitted manuscript or with a company making a competing product. Such information will be held in confidence while the paper is under review and will not influence the editorial decision, but if the article is accepted for publication, the editors will usually discuss with the authors the manner in which such information is to be communicated to the reader.
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