Bilin-regulated LHCA1 accumulation is independent of photoreceptors PHOT, CRYs, and UVR8 in Chlamydomonas reinhardtii

IF 5.9 3区工程技术 Q1 AGRONOMY

Global Change Biology Bioenergy Pub Date : 2024-05-31 DOI:10.1111/gcbb.13168

Chunhui Hou, Weiqing Zhang, Rui Deng, Hui Xiong, Deqiang Duanmu

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Abstract

Light is a critical environmental signal that is perceived by various photoreceptors and is of great significance in the photosynthetic growth of algae and plants. The phytochrome-lacking model green alga Chlamydomonas reinhardtii possesses heme oxygenase (HMOX1) and phycocyanobilin ferredoxin oxidoreductase (PCYA1) to synthesize the linear tetrapyrrole bilin from heme in the chloroplast. The hmox1 mutant has photosynthetic growth deficiency and accumulation of photosystem I proteins such as LHCA1 is severely inhibited, and these defects could be rescued by exogenous bilin feeding in a blue light-dependent manner. To investigate the contribution of the typical blue/ultraviolet light photoreceptors PHOT, aCRY, pCRY, and UVR8 in the process of bilin and blue light-dependent recovery of LHCA1 protein in hmox1, we generated double mutants of these photoreceptors in hmox1, as well as a triple mutant of phot uvr8 hmox1, to analyze the LHCA1 protein abundance in these mutants. Results clearly showed that PHOT, CRYs, and UVR8 do not participate in this process. In addition, transcriptome profiling analysis of the hmox1 and its genetically complemented strain ho1C2 during dark-to-blue light transition revealed a total of 269 blue light-responsive genes independent of bilin (|fold change| ≥ 2). RNA-seq also identified a set of 249 differentially expressed genes that are dependent on both blue light and bilin. These findings provide valuable insights for elucidating the role of bilin in mediating blue light signaling pathways in Chlamydomonas.

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Bilin 调节的 LHCA1 积累与衣藻中的感光器 PHOT、CRYs 和 UVR8 无关

光是一种重要的环境信号，可被各种光感受器感知，对藻类和植物的光合生长具有重要意义。缺乏植物色素的模式绿藻莱茵衣藻（Chlamydomonas reinhardtii）具有血红素加氧酶（HMOX1）和藻蓝蛋白铁氧还蛋白氧化还原酶（PCYA1），可在叶绿体中由血红素合成线性四吡咯双蛋白。hmox1 突变体存在光合生长缺陷，光系统 I 蛋白（如 LHCA1）的积累受到严重抑制，而这些缺陷可以通过外源比林喂养以蓝光依赖的方式得到挽救。为了研究典型的蓝光/紫外光光感受器PHOT、aCRY、pCRY和UVR8在bilin和蓝光依赖性恢复hmox1中LHCA1蛋白的过程中的贡献，我们在hmox1中产生了这些光感受器的双突变体，以及phot uvr8 hmox1的三突变体，分析这些突变体中LHCA1蛋白的丰度。结果清楚地表明，PHOT、CRYs 和 UVR8 并不参与这一过程。此外，对hmox1及其基因互补株ho1C2在暗光到蓝光转换过程中的转录组图谱分析表明，共有269个蓝光响应基因与bilin无关（|fold change| ≥ 2）。此外，RNA-seq 还发现了 249 个同时依赖于蓝光和 bilin 的差异表达基因。这些发现为阐明 bilin 在介导衣藻蓝光信号通路中的作用提供了宝贵的见解。

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来源期刊

Global Change Biology Bioenergy AGRONOMY-ENERGY & FUELS

CiteScore

10.30

自引率

7.10%

发文量

审稿时长

1.5 months

期刊介绍： GCB Bioenergy is an international journal publishing original research papers, review articles and commentaries that promote understanding of the interface between biological and environmental sciences and the production of fuels directly from plants, algae and waste. The scope of the journal extends to areas outside of biology to policy forum, socioeconomic analyses, technoeconomic analyses and systems analysis. Papers do not need a global change component for consideration for publication, it is viewed as implicit that most bioenergy will be beneficial in avoiding at least a part of the fossil fuel energy that would otherwise be used. Key areas covered by the journal: Bioenergy feedstock and bio-oil production: energy crops and algae their management,, genomics, genetic improvements, planting, harvesting, storage, transportation, integrated logistics, production modeling, composition and its modification, pests, diseases and weeds of feedstocks. Manuscripts concerning alternative energy based on biological mimicry are also encouraged (e.g. artificial photosynthesis). Biological Residues/Co-products: from agricultural production, forestry and plantations (stover, sugar, bio-plastics, etc.), algae processing industries, and municipal sources (MSW). Bioenergy and the Environment: ecosystem services, carbon mitigation, land use change, life cycle assessment, energy and greenhouse gas balances, water use, water quality, assessment of sustainability, and biodiversity issues. Bioenergy Socioeconomics: examining the economic viability or social acceptability of crops, crops systems and their processing, including genetically modified organisms [GMOs], health impacts of bioenergy systems. Bioenergy Policy: legislative developments affecting biofuels and bioenergy. Bioenergy Systems Analysis: examining biological developments in a whole systems context.