Transcription profiling reveals tissue-specific metabolic pathways in the fat body and ovary of the diapausing mosquito Culex pipiens

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Xueyan Wei , Karina Lee , Neha Mullassery , Prabin Dhungana , David S. Kang , Cheolho Sim
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Abstract

The northern house mosquito, Culex pipiens, employs diapause as an essential survival strategy during winter, inducing important phenotypic changes such as enhanced stress tolerance, lipid accumulation, and extended longevity. During diapause, the cessation of reproductive development represents another distinctive phenotypic change, underlining the need for adjusted modulation of gene expressions within the ovary. Although considerable advancements in screening gene expression profiles in diapausing and non-diapausing mosquitoes, there remains a gap in tissue-specific transcriptomic profiling that could elucidate the complicated formation of diverse diapause features in Cx. pipiens. Here, we filled this gap by utilizing RNA sequencing, providing a detailed examination of gene expression patterns in the fat body and ovary during diapause compared to non-diapause conditions. Functional annotation of upregulated genes identified associations with carbohydrate metabolism, stress tolerance, immunity, and epigenetic regulation. The validation of candidate genes using quantitative real-time PCR verified the differentially expressed genes identified in diapausing mosquitoes. Our findings contribute novel insights into potential regulators during diapause in Cx. pipiens, thereby opening possible avenues for developing innovative vector control strategies.

Abstract Image

转录剖析揭示了致病库蚊脂肪体和卵巢中特定组织的代谢途径。
北方家蚊(Culex pipiens)在冬季将休眠作为一种重要的生存策略,诱导重要的表型变化,如增强应激耐受性、脂质积累和延长寿命。在休眠期,生殖发育的停止代表了另一种独特的表型变化,强调了调整卵巢内基因表达的必要性。尽管在筛选停歇和非停歇蚊子的基因表达谱方面取得了相当大的进展,但在组织特异性转录组谱方面仍然存在空白,而这种转录组谱可以阐明蝰蛇不同停歇特征的复杂形成过程。在这里,我们利用 RNA 测序填补了这一空白,详细研究了与非休眠状态相比,脂肪体和卵巢在休眠状态下的基因表达模式。上调基因的功能注释确定了与碳水化合物代谢、应激耐受性、免疫和表观遗传调控有关的基因。使用定量实时 PCR 对候选基因进行了验证,证实了在绝食蚊子中发现的差异表达基因。我们的研究结果有助于深入了解喙蚊休眠期的潜在调节因子,从而为制定创新的病媒控制策略开辟了可能的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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