Heavy metal ions interactions with G-quadruplex-prone DNA sequences

IF 3.3 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Seyyed-Ali Mehrdad , Anne Cucchiarini , Jean-Louis Mergny , Sakineh Kazemi Noureini
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Abstract

The industrial world exposes living organisms to a variety of metal pollutants. Here we investigated whether such elements affect G-rich sequences susceptible to fold into G-quadruplex (GQ) structures. Thermal stability and conformation of these oligoncleotides was studied at various molar ratios of a variety of heavy metal salts using thermal FRET, transition-FRET (t-FRET) and circular dichroism. Metal ions affected the thermal stability of the GQs to different extents; some metals had no effect on Tm while other metals caused small to moderate changes in Tm at 1:1 or 1:10 molar ratio. While most of the metals had no major effect, Al3+, Cd2+, Pb2+, Hg2+ and Zn2+ altered the thermal stability and structural features of the GQs. Some metals such as Pb2+ and Hg2+ exhibit differential interactions with telomere, c-myc and c-kit GQs. Overall, toxic heavy metals affect G-quadruplex stability in a sequence and topology dependent manner. This study provides new insight into how heavy metal exposure may affect gene expression and cellular responses.

重金属离子与易发生 G 型四联DNA 序列的相互作用。
工业世界使生物体暴露在各种金属污染物中。在这里,我们研究了这些元素是否会影响容易折叠成 G-四重链(GQ)结构的富 G 序列。我们利用热 FRET、过渡-FRET(t-FRET)和圆二色性研究了这些寡核苷酸在各种重金属盐的不同摩尔比下的热稳定性和构象。金属离子对 GQ 的热稳定性有不同程度的影响;一些金属对 Tm 没有影响,而其他金属在摩尔比为 1:1 或 1:10 时会导致 Tm 发生小到中等程度的变化。虽然大多数金属没有大的影响,但 Al3+、Cd2+、Pb2+、Hg2+ 和 Zn2+ 会改变 GQs 的热稳定性和结构特征。一些金属如 Pb2+ 和 Hg2+ 与端粒、c-myc 和 c-kit GQs 的相互作用存在差异。总之,有毒重金属以序列和拓扑依赖的方式影响 G-四叉链的稳定性。这项研究为了解重金属暴露如何影响基因表达和细胞反应提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biochimie
Biochimie 生物-生化与分子生物学
CiteScore
7.20
自引率
2.60%
发文量
219
审稿时长
40 days
期刊介绍: Biochimie publishes original research articles, short communications, review articles, graphical reviews, mini-reviews, and hypotheses in the broad areas of biology, including biochemistry, enzymology, molecular and cell biology, metabolic regulation, genetics, immunology, microbiology, structural biology, genomics, proteomics, and molecular mechanisms of disease. Biochimie publishes exclusively in English. Articles are subject to peer review, and must satisfy the requirements of originality, high scientific integrity and general interest to a broad range of readers. Submissions that are judged to be of sound scientific and technical quality but do not fully satisfy the requirements for publication in Biochimie may benefit from a transfer service to a more suitable journal within the same subject area.
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