Antimicrobial resistance and genetic relatedness among Campylobacter coli and Campylobacter jejuni from humans and retail chicken meat in Taiwan

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Hsiao-Lun Wei, Ying-Shu Liao, Bo-Han Chen, Ru-Hsiou Teng, You-Wun Wang, Jui-Hsien Chang, Chien-Shun Chiou
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引用次数: 0

Abstract

Objectives

Campylobacter is a significant zoonotic pathogen primarily transmitted through poultry. Our study aimed to assess antimicrobial resistance and genetic relationships among Campylobacter isolates from retail chicken meat and humans in Taiwan.

Methods

Campylobacter isolates were analysed using whole-genome sequencing to investigate their antimicrobial resistance, genetic determinants of resistance, and genotypes.

Results

Campylobacter coli and Campylobacter jejuni accounted for 44.9% and 55.1% of chicken meat isolates, and 11.4% and 88.6% of human isolates, respectively. C. coli displayed significantly higher resistance levels. Furthermore, isolates from chicken meat exhibited higher levels of resistance to most tested antimicrobials compared to isolates from humans. Multidrug resistance was observed in 96.3% of C. coli and 43.3% of C. jejuni isolates from chicken meat and 80.6% of C. coli and 15.8% of C. jejuni isolates from humans. Macrolide resistance was observed in 85.5% of C. coli isolates, primarily attributed to the erm(B) rather than the A2075G mutation in 23S rRNA. Among the 511 genomes, we identified 133 conventional MLST sequence types, indicating significant diversity among Campylobacter strains. Notably, hierarchical Core-genome multilocus sequence typing clustering, including HC0, HC5, and HC10, revealed a significant proportion of closely related isolates from chicken meat and humans.

Conclusions

Our research highlights significant associations in antimicrobial resistance and genetic relatedness between Campylobacter isolates from chicken meat and humans in Taiwan. The genetic analysis data suggest that campylobacteriosis outbreaks may occur more frequently in Taiwan than previously assumed. Our study emphasizes the need for strategies to control multidrug-resistant strains and enhance outbreak prevention.

台湾地区人类和零售鸡肉中大肠弯曲杆菌和空肠弯曲杆菌的抗菌药耐药性和遗传亲缘关系。
目的:弯曲杆菌是一种重要的人畜共患病原体,主要通过家禽传播。我们的研究旨在评估来自台湾零售鸡肉和人类的弯曲杆菌分离物的抗菌性和遗传关系:方法:使用全基因组测序分析弯曲杆菌分离物,研究其抗菌性、抗菌性遗传决定因素和基因型:结果:大肠弯曲菌和空肠弯曲菌分别占鸡肉分离物的 44.9% 和 55.1%,占人类分离物的 11.4% 和 88.6%。大肠杆菌的耐药性水平明显更高。此外,与来自人类的分离物相比,来自鸡肉的分离物对大多数测试过的抗菌药物表现出更高的耐药性。在 96.3% 的鸡肉大肠杆菌和 43.3% 的空肠大肠杆菌分离物中,以及在 80.6% 的鸡肉大肠杆菌和 15.8% 的人类空肠大肠杆菌分离物中,都观察到了对多种药物的耐药性。在 85.5% 的大肠杆菌分离物中观察到了大环内酯耐药性,这主要归因于 23S rRNA 中的 erm(B) 突变而非 A2075G 突变。在 511 个基因组中,我们发现了 133 种常规 MLST 序列类型(ST),表明弯曲杆菌菌株之间存在显著的多样性。值得注意的是,包括 HC0、HC5 和 HC10 在内的 cgMLST 分层聚类显示,来自鸡肉和人类的分离菌株中有相当大比例的菌株密切相关:我们的研究突显了台湾鸡肉和人类弯曲杆菌分离物之间在抗菌药耐药性和遗传亲缘性方面的重要关联。基因分析数据表明,弯曲杆菌病在台湾的爆发频率可能比之前假设的更高。我们的研究强调了控制耐多药菌株和加强疫情预防策略的必要性。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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