Paired CRISPR screening libraries for studying the function of the non-coding genome at scale

IF 26.8 1区医学 Q1 ENGINEERING, BIOMEDICAL

Nature Biomedical Engineering Pub Date : 2024-05-31 DOI:10.1038/s41551-024-01215-5

引用次数: 0

Abstract

The majority of the human non-coding genome remains poorly studied. A user-friendly genome-wide screening system composed of thousands of paired single-guide RNAs for the deletion of non-coding regions revealed key functions of many non-coding elements in cell growth and cell differentiation and in cellular response to drugs.

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用于大规模研究非编码基因组功能的成对 CRISPR 筛选文库

对人类非编码基因组的大部分研究仍然很少。由数千条配对单导 RNA 组成的用户友好型全基因组筛选系统用于删除非编码区，该系统揭示了许多非编码元件在细胞生长、细胞分化以及细胞对药物反应中的关键功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Biomedical Engineering Medicine-Medicine (miscellaneous)

CiteScore

45.30

自引率

1.10%

发文量

138

期刊介绍： Nature Biomedical Engineering is an online-only monthly journal that was launched in January 2017. It aims to publish original research, reviews, and commentary focusing on applied biomedicine and health technology. The journal targets a diverse audience, including life scientists who are involved in developing experimental or computational systems and methods to enhance our understanding of human physiology. It also covers biomedical researchers and engineers who are engaged in designing or optimizing therapies, assays, devices, or procedures for diagnosing or treating diseases. Additionally, clinicians, who make use of research outputs to evaluate patient health or administer therapy in various clinical settings and healthcare contexts, are also part of the target audience.