Akkermansia muciniphila postbiotic administration mitigates choline-induced plasma Trimethylamine-N-Oxide production in mice

IF 2.3 3区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Applied Biological Chemistry Pub Date : 2024-05-30 DOI:10.1186/s13765-024-00905-7

Hongjuan Xu, Xiaoyun Bian, Hongxing Wang, Lin Huang, Xiaoxi Chen

{"title":"Akkermansia muciniphila postbiotic administration mitigates choline-induced plasma Trimethylamine-N-Oxide production in mice","authors":"Hongjuan Xu, Xiaoyun Bian, Hongxing Wang, Lin Huang, Xiaoxi Chen","doi":"10.1186/s13765-024-00905-7","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Trimethylamine-N-Oxide (TMAO) is believed to be linked to increased likelihood of cardiovascular disease. While probiotics have shown limited effectiveness in reducing TMAO levels, the potential of postbiotics remains underexplored. This study aimed to evaluate the impact of <i>Akkermansia muciniphila</i> (<i>A. muciniphila</i>) postbiotic administration on choline-induced TMAO production in mice by modifying the gut microbiota.</p><h3>Methods</h3><p>Female C57BL/6J mice were divided into six groups, including a control group, high-choline diet group, live <i>A. muciniphila</i> probiotic group, pasteurized <i>A. muciniphila</i> postbiotic group, sodium butyrate group, and sodium propionate group. Various measurements and analyses were conducted, including TMAO and TMA levels in serum, urine, and cecal contents, as well as the expression of FXR and FMO3 in liver tissues. Additionally, metabolic parameters, body weight, serum lipid profile, hepatic protein expression (FMO3, FXR, CutC, and CutD), and gut microbiota composition were assessed.</p><h3>Results</h3><p>Administration of <i>A. muciniphila</i> postbiotic significantly reduced choline-induced plasma TMAO levels in mice. Furthermore, improvements in serum lipid profiles and liver enzyme levels suggested potential enhancements in lipid metabolism and liver function. The study also observed modulation of specific proteins related to TMAO production and metabolism, including CutC and CutD.</p><h3>Conclusion</h3><p>The findings highlight the potential of <i>A. muciniphila</i> postbiotics as a dietary strategy for mitigating cardiovascular disease risk by modulating the gut-TMAO axis. Postbiotics, particularly <i>A. muciniphila</i>, offer advantages over probiotics and warrant further investigation for their therapeutic applications in gastrointestinal and metabolic disorders.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00905-7","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biological Chemistry","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s13765-024-00905-7","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Trimethylamine-N-Oxide (TMAO) is believed to be linked to increased likelihood of cardiovascular disease. While probiotics have shown limited effectiveness in reducing TMAO levels, the potential of postbiotics remains underexplored. This study aimed to evaluate the impact of Akkermansia muciniphila (A. muciniphila) postbiotic administration on choline-induced TMAO production in mice by modifying the gut microbiota.

Methods

Female C57BL/6J mice were divided into six groups, including a control group, high-choline diet group, live A. muciniphila probiotic group, pasteurized A. muciniphila postbiotic group, sodium butyrate group, and sodium propionate group. Various measurements and analyses were conducted, including TMAO and TMA levels in serum, urine, and cecal contents, as well as the expression of FXR and FMO3 in liver tissues. Additionally, metabolic parameters, body weight, serum lipid profile, hepatic protein expression (FMO3, FXR, CutC, and CutD), and gut microbiota composition were assessed.

Results

Administration of A. muciniphila postbiotic significantly reduced choline-induced plasma TMAO levels in mice. Furthermore, improvements in serum lipid profiles and liver enzyme levels suggested potential enhancements in lipid metabolism and liver function. The study also observed modulation of specific proteins related to TMAO production and metabolism, including CutC and CutD.

Conclusion

The findings highlight the potential of A. muciniphila postbiotics as a dietary strategy for mitigating cardiovascular disease risk by modulating the gut-TMAO axis. Postbiotics, particularly A. muciniphila, offer advantages over probiotics and warrant further investigation for their therapeutic applications in gastrointestinal and metabolic disorders.

Graphical Abstract

查看原文本刊更多论文

给小鼠服用 Akkermansia muciniphila 益生菌后可减轻胆碱诱导的血浆三甲胺氧化物的产生

背景三甲胺氧化物（TMAO）被认为与心血管疾病的发病率增加有关。虽然益生菌在降低 TMAO 水平方面的效果有限，但后益生菌的潜力仍未得到充分开发。方法将雌性 C57BL/6J 小鼠分为六组，包括对照组、高胆碱饮食组、活粘液素益生菌组、巴氏粘液素益生菌组、丁酸钠组和丙酸钠组。进行了各种测量和分析，包括血清、尿液和盲肠内容物中的 TMAO 和 TMA 含量，以及肝组织中 FXR 和 FMO3 的表达。此外，还评估了代谢参数、体重、血清脂质概况、肝脏蛋白质表达（FMO3、FXR、CutC 和 CutD）以及肠道微生物群组成。此外，血清脂质概况和肝酶水平的改善表明，脂质代谢和肝功能有可能得到改善。研究还观察到与 TMAO 生成和代谢有关的特定蛋白质（包括 CutC 和 CutD）受到了调节。与益生菌相比，后益生菌，尤其是粘液虹鳟具有优势，值得进一步研究其在胃肠道和代谢疾病中的治疗应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Applied Biological Chemistry Chemistry-Organic Chemistry

CiteScore

5.40

自引率

6.20%

发文量

审稿时长

20 weeks

期刊介绍： Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.