Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins.

IF 14 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Trends in Microbiology Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI:10.1016/j.tim.2024.05.004

Zhenkun Cai, Weiyu Xie, Zehua Bao

引用次数: 0

Abstract

The application of CRISPR-Cas systems has been hindered by their requirement for long protospacer-adjacent motifs (PAMs). Recent engineering and discovery of PAM-flexible Cas proteins have substantially broadened the targetable DNA sequence space, thereby facilitating genome editing and improving derivative technologies such as gene regulation, seamless cloning, and large-scale genetic screens.

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拓宽靶向空间：PAM 灵活 Cas 蛋白的工程设计与发现。

CRISPR-Cas系统的应用一直受制于对长原位相邻基序（PAM）的要求。最近，PAM 灵活 Cas 蛋白的工程设计和发现大大拓宽了可靶向的 DNA 序列空间，从而促进了基因组编辑，并改善了基因调控、无缝克隆和大规模基因筛选等衍生技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Microbiology 生物-生化与分子生物学

CiteScore

25.30

自引率

0.60%

发文量

193

审稿时长

6-12 weeks

期刊介绍： Trends in Microbiology serves as a comprehensive, multidisciplinary forum for discussing various aspects of microbiology, spanning cell biology, immunology, genetics, evolution, virology, bacteriology, protozoology, and mycology. In the rapidly evolving field of microbiology, technological advancements, especially in genome sequencing, impact prokaryote biology from pathogens to extremophiles, influencing developments in drugs, vaccines, and industrial enzyme research.