Design of Polymeric Nanoparticles for Theranostic Delivery of Capsaicin as Anti-Cancer Drug and Fluorescent Nitrogen-Doped Graphene Quantum Dots

IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Berrin Küçüktürkmen, Umut Can Öz, Engin Er, I. Jénnifer Gómez, Seda İpek Tekneci, Özgür Eşim, Umut Uğur Özköse, Sevgi Gülyüz, Aylin Üstündağ, Özgür Yılmaz, Lenka Zajíčková, Asuman Bozkır
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Abstract

In recent years, multifunctional nanocarriers that provide simultaneous drug delivery and imaging have attracted enormous attention, especially in cancer treatment. In this research, a biocompatible fluorescent multifunctional nanocarrier is designed for the co-delivery of capsaicin (CPS) and nitrogen-doped graphene quantum dots (N-GQDs) using the pH sensitive amphiphilic block copolymer (poly(2-ethyl-2-oxazoline)-b-poly(ε-caprolactone), PEtOx-b-PCL). The effects of the critical formulation parameters (the amount of copolymer, the concentration of poly(vinyl alcohol) (PVA) as a stabilizing agent in the inner aqueous phase, and volume of the inner phase) are evaluated to achieve optimal nanoparticle (NP) properties using Central Composite Design. The optimized NPs demonstrated a desirable size distribution (167.8 ± 1.4 nm) with a negative surface charge (−19.9 ± 0.4) and a suitable loading capacity for CPS (70.80 ± 0.05%). The CPS & N-GQD NPs are found to have remarkable toxicity on human breast adenocarcinoma cell line (MCF-7). The solid fluorescent signal is acquired from cells containing multifunctional NPs, according to the confocal microscope imaging results, confirming the significant cellular uptake. This research illustrates the enormous potential for cellular imaging and enhanced cancer therapy offered by multifunctional nanocarriers that combine drug substances with the novel fluorescent agents.

Abstract Image

设计抗癌药物辣椒素和掺杂荧光氮的石墨烯量子点的纳米聚合物颗粒。
近年来,能够同时实现药物输送和成像的多功能纳米载体引起了广泛关注,尤其是在癌症治疗领域。在这项研究中,我们利用 pH 敏感的两亲嵌段共聚物(聚(2-乙基-2-噁唑啉)-b-聚(ε-己内酰胺),PEtOx-b-PCL)设计了一种生物相容性荧光多功能纳米载体,用于辣椒素(CPS)和氮掺杂石墨烯量子点(N-GQDs)的联合给药。利用中央复合设计(CCD)评估了关键配方参数(共聚物的用量、内水相中作为稳定剂的聚(乙烯醇)(PVA)的浓度和内相的体积)的影响,以获得最佳的纳米粒子特性。优化后的纳米粒子具有理想的尺寸分布(167.8±1.4 nm)和负表面电荷(-19.9±0.4),并具有合适的辣椒素负载能力(70.80±0.05%)。研究发现,CPS 和 N-GQD NPs 对人类乳腺癌细胞株(MCF-7)具有显著的毒性。根据共聚焦显微镜成像结果,含有多功能纳米粒子的细胞获得了固体荧光信号,证实了细胞的显著吸收。这项研究表明,将药物物质与新型荧光剂结合在一起的多功能纳米载体在细胞成像和增强癌症治疗方面具有巨大潜力。本文受版权保护。保留所有权利。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
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