Expression and Mutation of SLC45A2 Affects Iris Color in Quail.

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Poultry Science Pub Date : 2024-05-30 eCollection Date: 2024-01-01 DOI:10.2141/jpsa.2024015
Linke Huo, Xiaohui Zhang, Youzhi Pang, Yanxia Qi, Shiwei Ren, Fanghu Wu, Yuanyuan Shang, Jinquan Xi
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引用次数: 0

Abstract

Iris color is a prominent phenotypic feature of quail. To understand the mechanism of melanin deposition related to quail iris color, iris tissues were selected from Beijing white and Chinese yellow quail for transcriptome analysis. Differentially expressed genes (DEGs) associated with pigmentation were identified using RNA sequencing and validated by quantitative real-time polymerase chain reaction (RT-qPCR). The identified single nucleotide polymorphisms were studied using bioinformatics and iris color correlation analyses. A total of 485 DEGs were obtained, with 223 upregulated and 262 downregulated. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed. Thirty-two genes were annotated using the GO database. Three important pigment synthesis pathways (Notch signaling, melanogenesis, and tyrosine metabolism) were identified in quail iris tissue (P < 0.05). The expression levels of solute carrier family 45 member 2 (SLC45A2), tyrosinase-related protein 1, vitamin D receptor, opsin 5, and docking protein 5 were significantly different between Beijing white and Chinese yellow quail, as verified by RT-qPCR. The c.1061C>T mutation in SLC45A2, which caused a single amino acid change at position 354 (threonine to methionine), was significantly associated with iris color in Beijing white and Chinese yellow quail, and might be the main reason for the different iris colors between these two quail species.

SLC45A2 的表达和突变影响鹌鹑虹膜的颜色
虹膜颜色是鹌鹑的一个显著表型特征。为了解鹌鹑虹膜颜色的黑色素沉积机制,研究人员选取了北京白鹌鹑和中国黄鹌鹑的虹膜组织进行转录组分析。利用 RNA 测序鉴定了与色素沉着相关的差异表达基因(DEGs),并通过实时定量聚合酶链反应(RT-qPCR)进行了验证。利用生物信息学和虹膜颜色相关性分析对已确定的单核苷酸多态性进行了研究。共获得 485 个 DEGs,其中上调 223 个,下调 262 个。进行了基因本体(GO)和京都基因组百科全书通路富集分析。利用 GO 数据库对 32 个基因进行了注释。在鹌鹑虹膜组织中发现了三个重要的色素合成通路(Notch 信号转导、黑色素生成和酪氨酸代谢)(P < 0.05)。经 RT-qPCR 验证,溶质运载家族 45 成员 2(SLC45A2)、酪氨酸酶相关蛋白 1、维生素 D 受体、眼色素 5 和对接蛋白 5 的表达水平在北京白鹑和中国黄鹑之间存在显著差异。SLC45A2的c.1061C>T突变导致354位的单个氨基酸变化(苏氨酸变为蛋氨酸),与北京白鹑和中国黄鹑的虹膜颜色显著相关,可能是造成这两种鹌鹑虹膜颜色不同的主要原因。
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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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