Optimization, characterization and evaluation of sodium alginate nanoparticles for Ganoderic acid DM encapsulation: Inhibitory activity on tyrosinase activity and melanin formation

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2024-06-01 DOI:10.1016/j.ijbiomac.2024.132717

Shu Luo, Yi Song, Zhou Zhou, Xiao-yan Xu, Nan Jiang, Ying-juan Gao, Xia Luo

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引用次数: 0

Abstract

The efficacy of nanoencapsulation as a technology for enhancing the solubility of active substances has been demonstrated. In this particular investigation, Ganoderic acid DM (GA-DM) was encapsulated within sodium alginate nanoparticles (NPs) using the ionic crosslinking method. The confirmation of the successful loading of GA-DM was ascertained through the analysis of Fourier transform infrared spectrum (FTIR). Empirical evidence derived from the examination of scanning electron microscope (SEM) images, transmission electron microscope (TEM) images, atomic force microscope (AFM) images, and dynamic light scattering (DLS) demonstrated a regular distribution and spherical morphology, with an average particle size of approximately 133 nm. The investigation yielded an encapsulation efficiency of 95.27 ± 0.11 % and a drug loading efficiency of 21.17 ± 0.02 % for the prepared sample. The release kinetics of SGPN was fitted with the Korsmeyer-Peppas kinetic model corresponding to diffusion-controlled release. The incorporation of GA-DM into sodium alginate nanocarriers exhibited a mitigating effect on the cytotoxicity of HaCat and B16, while also demonstrating inhibitory properties against tyrosinase activity and melanin formation.

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海藻酸钠纳米颗粒在甘草酸 DM 包封中的优化、表征和评估：对酪氨酸酶活性和黑色素形成的抑制活性。

纳米封装作为一种提高活性物质溶解度的技术，其功效已得到证实。在这项特定的研究中，利用离子交联法在海藻酸钠纳米粒子（NPs）中封装了灵芝酸 DM（GA-DM）。通过分析傅立叶变换红外光谱（FTIR）确认了 GA-DM 的成功负载。通过扫描电子显微镜（SEM）图像、透射电子显微镜（TEM）图像、原子力显微镜（AFM）图像和动态光散射（DLS）检查得出的经验证据表明，GA-DM 具有规则的分布和球形形态，平均粒径约为 133 nm。研究结果表明，所制备样品的封装效率为 95.27 ± 0.11 %，药物负载效率为 21.17 ± 0.02 %。SGPN 的释放动力学符合 Korsmeyer-Peppas 动力学模型，即扩散控制释放模型。在海藻酸钠纳米载体中加入 GA-DM 可减轻 HaCat 和 B16 的细胞毒性，同时还具有抑制酪氨酸酶活性和黑色素形成的特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.

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sodium alginate