Phenotypic and Genetic Diversity of Xanthomonads Isolated from Pepper (Capsicum spp.) in Taiwan from 1989 to 2019.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-09-01 Epub Date: 2024-09-16 DOI:10.1094/PHYTO-11-23-0449-R

Apekshya Parajuli, Aastha Subedi, Sujan Timilsina, Gerald V Minsavage, Lawrence Kenyon, Jaw-Rong Chen, Erica M Goss, Mathews L Paret, Jeffrey B Jones

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引用次数: 0

Abstract

Bacterial spot caused by Xanthomonas spp. is an economically important disease of pepper causing significant yield losses in Taiwan. Monitoring the pathogen population on a continuous basis is necessary for developing disease management strategies. We analyzed a collection of xanthomonad strains isolated from pepper in Taiwan between 1989 and 2019. Among the sequenced genomes, 65 were identified as Xanthomonas euvesicatoria, and 10 were X. perforans. Thirty-five X. euvesicatoria and 10 X. perforans strains were copper tolerant, whereas only four X. euvesicatoria and none of the X. perforans strains were tolerant to streptomycin. Nine X. euvesicatoria strains were amylolytic, which is considered an unusual characteristic for X. euvesicatoria. Bayesian analysis of the population structure based on core gene single-nucleotide polymorphisms clustered the strains into five clusters for X. euvesicatoria and three clusters for X. perforans. One X. perforans cluster, designated as TP-2019, appears to be a novel genetic cluster based on core genes, accessory gene content, and effector profile. This knowledge of pathogen diversity with whole genomic information will be useful in future comparative studies and in improving breeding programs to develop disease-resistant cultivars and other disease management options.

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1989年至2019年从台湾辣椒中分离的黄单胞菌的表型和遗传多样性。

由黄单胞菌属引起的细菌性斑点病是辣椒的一种重要经济病害，在台湾造成严重的产量损失。要制定病害管理策略，就必须持续监测病原菌种群。我们分析了1989年至2019年期间从台湾辣椒中分离的黄单胞菌菌株。在已测序的基因组中，65株被鉴定为黄单胞菌（Xanthomonas euvesicatoria），10株为X.35株X. euvesicatoria和10株X. perforans耐铜，而只有5株X. euvesicatoria和没有一株X. perforans耐链霉素。9 株 X. euvesicatoria 具有淀粉溶解性，这被认为是 X. euvesicatoria 的一个不寻常特征。基于核心基因 SNPs 的种群结构贝叶斯分析将 X. euvesicatoria 菌株分为五个群，X. perforans 菌株分为三个群。其中一个 X. perforans 聚类（命名为 TP-2019）似乎是一个基于核心基因、附属基因含量和效应物特征的新型基因聚类。利用全基因组信息了解病原体的多样性将有助于未来的比较研究和改进育种计划，以开发抗病栽培品种和其他病害管理方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.