Two Distinct Enzymes Have Both Phytoene Desaturase and 3,4-Desaturase Activities Involved in Carotenoid Biosynthesis by the Extremely Halophilic Archaeon Haloarcula japonica.

IF 2.1 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2024-01-01 DOI:10.1264/jsme2.ME24004

Rie Yatsunami, Ai Ando, Nobuhiro Miyoko, Ying Yang, Shinichi Takaichi, Satoshi Nakamura

{"title":"Two Distinct Enzymes Have Both Phytoene Desaturase and 3,4-Desaturase Activities Involved in Carotenoid Biosynthesis by the Extremely Halophilic Archaeon Haloarcula japonica.","authors":"Rie Yatsunami, Ai Ando, Nobuhiro Miyoko, Ying Yang, Shinichi Takaichi, Satoshi Nakamura","doi":"10.1264/jsme2.ME24004","DOIUrl":null,"url":null,"abstract":"The extremely halophilic archaeon Haloarcula japonica accumulates the C50 carotenoid, bacterioruberin (BR). To reveal the BR biosynthetic pathway, unidentified phytoene desaturase candidates were functionally characterized in the present study. Two genes encoding the potential phytoene desaturases, c0507 and d1086, were found from the Ha. japonica genome sequence by a homology search using the Basic Local Align Search Tool. Disruption mutants of c0507 and d1086 and their complemented strains transformed with expression plasmids for c0507 and d1086 were subsequently constructed. High-performance liquid chromatography (HPLC) ana-lyses of carotenoids produced by these strains revealed that C0507 and D1086 were both bifunctional enzymes with the same activities as both phytoene desaturase (CrtI) and 3,4-desaturase (CrtD). C0507 and D1086 complemented each other during BR biosynthesis in Ha. japonica. This is the first study to identify two distinct enzymes with both CrtI and CrtD activities in an extremely halophilic archaeon.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 5","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbes and Environments","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1264/jsme2.ME24004","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The extremely halophilic archaeon Haloarcula japonica accumulates the C₅₀ carotenoid, bacterioruberin (BR). To reveal the BR biosynthetic pathway, unidentified phytoene desaturase candidates were functionally characterized in the present study. Two genes encoding the potential phytoene desaturases, c0507 and d1086, were found from the Ha. japonica genome sequence by a homology search using the Basic Local Align Search Tool. Disruption mutants of c0507 and d1086 and their complemented strains transformed with expression plasmids for c0507 and d1086 were subsequently constructed. High-performance liquid chromatography (HPLC) ana-lyses of carotenoids produced by these strains revealed that C0507 and D1086 were both bifunctional enzymes with the same activities as both phytoene desaturase (CrtI) and 3,4-desaturase (CrtD). C0507 and D1086 complemented each other during BR biosynthesis in Ha. japonica. This is the first study to identify two distinct enzymes with both CrtI and CrtD activities in an extremely halophilic archaeon.

查看原文本刊更多论文

两种不同的酶同时具有植物烯去饱和酶和 3,4-去饱和酶的活性，它们参与了极嗜卤古菌 Haloarcula japonica 的类胡萝卜素生物合成。

嗜卤性极强的古菌 Haloarcula japonica 能积累 C50 类胡萝卜素--细菌素（BR）。为了揭示BR的生物合成途径，本研究对未确定的植物烯去饱和酶候选基因进行了功能鉴定。通过使用 "基本局部对齐搜索工具"（Basic Local Align Search Tool）进行同源性搜索，从 Ha. japonica 基因组序列中发现了两个编码潜在植物烯去饱和酶的基因：c0507 和 d1086。随后构建了 c0507 和 d1086 的中断突变体及其用 c0507 和 d1086 的表达质粒转化的互补菌株。对这些菌株产生的类胡萝卜素进行高效液相色谱分析（HPLC）发现，C0507 和 D1086 都是双功能酶，具有与植物烯去饱和酶（CrtI）和 3,4-去饱和酶（CrtD）相同的活性。C0507 和 D1086 在 Ha. japonica 的 BR 生物合成过程中具有互补作用。这是首次在一种极端嗜卤的古生物中发现两种同时具有CrtI和CrtD活性的不同酶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.