Repeated Exposure of Vancomycin to Vancomycin-Susceptible Staphylococcus aureus (VSSA) Parent Emerged VISA and VRSA Strains with Enhanced Virulence Potentials.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2024-07-01 Epub Date: 2024-05-30 DOI:10.1007/s12275-024-00139-8
An Nguyen, J Jean Sophy Roy, Ji-Hoon Kim, Kyung-Hee Yun, Wonsik Lee, Kyeong Kyu Kim, Truc Kim, Akhilesh Kumar Chaurasia
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Abstract

The emergence of resistance against the last-resort antibiotic vancomycin in staphylococcal infections is a serious concern for human health. Although various drug-resistant pathogens of diverse genetic backgrounds show higher virulence potential, the underlying mechanism behind this is not yet clear due to variability in their genetic dispositions. In this study, we investigated the correlation between resistance and virulence in adaptively evolved isogenic strains. The vancomycin-susceptible Staphylococcus aureus USA300 was exposed to various concentrations of vancomycin repeatedly as a mimic of the clinical regimen to obtain mutation(s)-accrued-clonally-selected (MACS) strains. The phenotypic analyses followed by expression of the representative genes responsible for virulence and resistance of MACS strains were investigated. MACS strains obtained under 2 and 8 µg/ml vancomycin, named Van2 and Van8, respectively; showed enhanced vancomycin minimal inhibitory concentrations (MIC) to 4 and 16 µg/ml, respectively. The cell adhesion and invasion of MACS strains increased in proportion to their MICs. The correlation between resistance and virulence potential was partially explained by the differential expression of genes known to be involved in both virulence and resistance in MACS strains compared to parent S. aureus USA300. Repeated treatment of vancomycin against vancomycin-susceptible S. aureus (VSSA) leads to the emergence of vancomycin-resistant strains with variable levels of enhanced virulence potentials.

Abstract Image

将万古霉素重复暴露于万古霉素易感金黄色葡萄球菌 (VSSA) 亲本,可产生具有更强毒性潜能的 VISA 和 VRSA 菌株。
葡萄球菌感染中出现的对最后一种抗生素万古霉素的耐药性是人类健康的一个严重问题。虽然不同基因背景的各种耐药病原体表现出更高的毒力潜力,但由于其基因处置的差异性,其背后的潜在机制尚不清楚。在本研究中,我们调查了适应性进化的同源菌株的耐药性和毒力之间的相关性。模仿临床治疗方案,将万古霉素易感的金黄色葡萄球菌 USA300 反复暴露于不同浓度的万古霉素,以获得突变-累积-克隆选择(MACS)菌株。研究人员对 MACS 菌株进行了表型分析,并对其毒力和耐药性的代表性基因表达进行了研究。在万古霉素2微克/毫升和8微克/毫升条件下获得的MACS菌株分别被命名为Van2和Van8;万古霉素最小抑菌浓度(MIC)分别提高到4微克/毫升和16微克/毫升。MACS 菌株的细胞粘附力和侵袭力与 MIC 值成正比。MACS 菌株与母体金黄色葡萄球菌 USA300 相比,已知参与毒力和耐药性的基因表达不同,这在一定程度上解释了耐药性与毒力之间的相关性。针对万古霉素易感金黄色葡萄球菌(VSSA)重复使用万古霉素会导致万古霉素耐药菌株的出现,而耐药菌株的毒力潜能会有不同程度的增强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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