Distinct properties of putative trophoblast stem cells established from somatic cell nuclear-transferred pig blastocysts.

IF 4.3 2区生物学 Q1 BIOLOGY

Biological Research Pub Date : 2024-05-30 DOI:10.1186/s40659-024-00516-y

Eunhye Kim, Lian Cai, Hyerin Choi, Mirae Kim, Sang-Hwan Hyun

{"title":"Distinct properties of putative trophoblast stem cells established from somatic cell nuclear-transferred pig blastocysts.","authors":"Eunhye Kim, Lian Cai, Hyerin Choi, Mirae Kim, Sang-Hwan Hyun","doi":"10.1186/s40659-024-00516-y","DOIUrl":null,"url":null,"abstract":"Background: Genetically modified pigs are considered ideal models for studying human diseases and potential sources for xenotransplantation research. However, the somatic cell nuclear transfer (SCNT) technique utilized to generate these cloned pig models has low efficiency, and fetal development is limited due to placental abnormalities.Results: In this study, we unprecedentedly established putative porcine trophoblast stem cells (TSCs) using SCNT and in vitro-fertilized (IVF) blastocysts through the activation of Wing-less/Integrated (Wnt) and epidermal growth factor (EGF) pathways, inhibition of transforming growth factor-β (TGFβ) and Rho-associated protein kinase (ROCK) pathways, and supplementation with ascorbic acid. We also compared the transcripts of putative TSCs originating from SCNT and IVF embryos and their differentiated lineages. A total of 19 porcine TSCs exhibiting typical characteristics were established from SCNT and IVF blastocysts (TSCsNT and TSCsIVF). Compared with the TSCsIVF, TSCsNT showed distinct expression patterns suggesting unique TSCsNT characteristics, including decreased mRNA expression of genes related to apposition, steroid hormone biosynthesis, angiopoiesis, and RNA stability.Conclusion: This study provides valuable information and a powerful model for studying the abnormal development and dysfunction of trophoblasts and placentas in cloned pigs.","PeriodicalId":9084,"journal":{"name":"Biological Research","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11137969/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s40659-024-00516-y","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Genetically modified pigs are considered ideal models for studying human diseases and potential sources for xenotransplantation research. However, the somatic cell nuclear transfer (SCNT) technique utilized to generate these cloned pig models has low efficiency, and fetal development is limited due to placental abnormalities.

Results: In this study, we unprecedentedly established putative porcine trophoblast stem cells (TSCs) using SCNT and in vitro-fertilized (IVF) blastocysts through the activation of Wing-less/Integrated (Wnt) and epidermal growth factor (EGF) pathways, inhibition of transforming growth factor-β (TGFβ) and Rho-associated protein kinase (ROCK) pathways, and supplementation with ascorbic acid. We also compared the transcripts of putative TSCs originating from SCNT and IVF embryos and their differentiated lineages. A total of 19 porcine TSCs exhibiting typical characteristics were established from SCNT and IVF blastocysts (TSCs^NT and TSCs^IVF). Compared with the TSCs^IVF, TSCs^NT showed distinct expression patterns suggesting unique TSCs^NT characteristics, including decreased mRNA expression of genes related to apposition, steroid hormone biosynthesis, angiopoiesis, and RNA stability.

Conclusion: This study provides valuable information and a powerful model for studying the abnormal development and dysfunction of trophoblasts and placentas in cloned pigs.

查看原文本刊更多论文

从体细胞核移植猪囊胚建立的假定滋养层干细胞的不同特性

背景：转基因猪被认为是研究人类疾病的理想模型，也是异种移植研究的潜在来源。然而，用于产生这些克隆猪模型的体细胞核移植（SCNT）技术效率较低，胎儿发育因胎盘异常而受到限制：在这项研究中，我们通过激活无翼/整合（Wnt）和表皮生长因子（EGF）通路、抑制转化生长因子-β（TGFβ）和Rho相关蛋白激酶（ROCK）通路以及补充抗坏血酸，利用SCNT和体外受精（IVF）囊胚史无前例地建立了猪滋养层干细胞（TSCs）。我们还比较了来源于SCNT和IVF胚胎的假定TSCs及其分化系的转录本。从 SCNT 和 IVF 胚泡中共建立了 19 个具有典型特征的猪 TSCs（TSCsNT 和 TSCsIVF）。与 TSCsIVF 相比，TSCsNT 显示出不同的表达模式，表明 TSCsNT 具有独特的特征，包括与贴壁、类固醇激素生物合成、血管生成和 RNA 稳定性相关的基因 mRNA 表达减少：这项研究为研究克隆猪滋养细胞和胎盘的异常发育和功能障碍提供了宝贵的信息和强大的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biological Research 生物-生物学

CiteScore

10.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biological Research is an open access, peer-reviewed journal that encompasses diverse fields of experimental biology, such as biochemistry, bioinformatics, biotechnology, cell biology, cancer, chemical biology, developmental biology, evolutionary biology, genetics, genomics, immunology, marine biology, microbiology, molecular biology, neuroscience, plant biology, physiology, stem cell research, structural biology and systems biology.