Live imaging analysis of sexual and asexual reproduction, zygospore and sporangiospore formation, in Gilbertella persicaria

IF 1.5 4区 生物学 Q4 MYCOLOGY
Norio Takeshita, Yusuke Takashima, Yousuke Degawa
{"title":"Live imaging analysis of sexual and asexual reproduction, zygospore and sporangiospore formation, in Gilbertella persicaria","authors":"Norio Takeshita, Yusuke Takashima, Yousuke Degawa","doi":"10.47371/mycosci.2024.03.002","DOIUrl":null,"url":null,"abstract":"</p><p>Most <i>Mucoromycota</i> fungi form zygospores as sexual reproductive structures. When two colonies of compatible strains meet, zygospores are formed in the area where the colonies meet. The structure and development of zygospores have been studied for a long time by light microscopy and electron microscopy. This study is the first time-lapse report on the dynamic movements of sexual and asexual reproductive processes by live imaging in <i>Gilbertella persicaria</i> (<i>Choanephoraceae, Mucorales</i>). Our live imaging analysis indicated the formation of zygospores begin immediately after two aerial hyphae contact whether at the tip or middle of the hyphae. The early-stage zygospores elongated from the contact site with a rate of 1.2–1.7 μm/s and reach &lt; 200 μm in 2–3 h. Following maturation of zygospores, from progametangia to gametangia and maturation stage, took a few hours, in total 5 to 6 h after the first contact of two hyphae. When a zygospore was formed near the tip of hypha in contact with the partner hypha, the hyphal growth ceased. When zygospore was formed behind the tip of the hypha, the hyphal growth continued without slowing down. This study provides quantitative spatio-temporal information on the dynamics of zygospore formation.</p>\n<p></p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycoscience","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.47371/mycosci.2024.03.002","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Most Mucoromycota fungi form zygospores as sexual reproductive structures. When two colonies of compatible strains meet, zygospores are formed in the area where the colonies meet. The structure and development of zygospores have been studied for a long time by light microscopy and electron microscopy. This study is the first time-lapse report on the dynamic movements of sexual and asexual reproductive processes by live imaging in Gilbertella persicaria (Choanephoraceae, Mucorales). Our live imaging analysis indicated the formation of zygospores begin immediately after two aerial hyphae contact whether at the tip or middle of the hyphae. The early-stage zygospores elongated from the contact site with a rate of 1.2–1.7 μm/s and reach < 200 μm in 2–3 h. Following maturation of zygospores, from progametangia to gametangia and maturation stage, took a few hours, in total 5 to 6 h after the first contact of two hyphae. When a zygospore was formed near the tip of hypha in contact with the partner hypha, the hyphal growth ceased. When zygospore was formed behind the tip of the hypha, the hyphal growth continued without slowing down. This study provides quantitative spatio-temporal information on the dynamics of zygospore formation.

对宿主吉尔伯特氏菌的有性生殖和无性生殖、子孢子和孢子囊形成的实时成像分析
大多数粘菌纲真菌都会形成子囊孢子作为有性生殖结构。当两个相容菌株的菌落相遇时,就会在菌落相遇的地方形成子囊孢子。长期以来,人们一直通过光学显微镜和电子显微镜研究子囊孢子的结构和发育。本研究首次通过活体成像技术,对柿树吉尔伯特菌(Choanephoraceae,Mucorales)有性生殖和无性生殖过程的动态运动进行了延时报道。我们的活体成像分析表明,无论是在菌丝的顶端还是中部,当两条气生菌丝接触后,子囊孢子立即开始形成。早期的子囊孢子以每秒 1.2-1.7 μm 的速度从接触部位开始伸长,2-3 小时后就能达到 < 200 μm。当子孢子在与伴侣菌丝接触的菌丝顶端附近形成时,菌丝生长停止。当子孢子在菌丝顶端后方形成时,菌丝生长继续,没有减缓。这项研究提供了关于子囊孢子形成动态的定量时空信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信