Evolution of the Antibacterial and Cellular Viability Effects of Silica-Hydroxyapatite Conjugated High Cerium Oxide Nanoparticles on Orthodontic Brackets

IF 4.7 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2024-05-28 DOI:10.1007/s10924-024-03325-2

Yanlei Kong, Feilong Zhang, Nan Zhou, Hongyu Zhang, Hao Wang

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Abstract

Nanomaterials have been utilized as antibacterial agents due to their distinct mechanism of action, which sets them apart from traditional antibiotics. Cerium oxide nanoparticles (CeO2 NPs) play a crucial role as antibacterial agents because of their minimal toxicity towards healthy cells and their unique antibacterial mechanism, which involves the reversible transition between two valence states of Ce(III)/Ce(IV). In the current study, silica microspheres (SiO₂) were applied for the growth of hydroxyapatite (Hap) over the silica surface. Then, the SiO₂/hydroxyapatite (SiO₂/Hap) nanocomposite was applied for stabilization of the in situ green synthesized cerium oxide nanoparticles (CeO₂ NPs) on its surface to assess the antibacterial and cellular viability of orthodontic brackets. The as-prepared SiO₂/Hap-CeO₂ NPs were fully characterized by different techniques such as FT-IR, XRD, FE-SEM, TEM and ICP-OES analyses. The technique employed to coat the orthodontic bands with SiO₂/Hap-CeO₂ NPs nanocomposite was the electrostatic spray-assisted vapor deposition method. The antibacterial efficacy of SiO₂/Hap-CeO₂ NPs nanocomposite against Candida albicans, Streptococcus mutans and Lactobacillus acidophilus was evaluated using the biofilm inhibition test. In the recent study’s cellular and molecular component, the treated cells with SiO₂/Hap-CeO₂ NPs nanocomposite underwent evaluation through MTT assay to determine cytotoxicity on the normal (HUVEC) cell line. In comparison to the control group, the SiO₂/Hap-CeO₂ NPs nanocomposite group exhibited a 2-log10 decrease in the replication ability of all microorganisms. The group of SiO₂/Hap-CeO₂ NPs nanocomposite demonstrated a notable decrease in the quantity of C. albicans, S. mutans and L. acidophilus colonies compared to the control group. Application of SiO₂/Hap-CeO₂ NPs nanocomposite on orthodontic bands resulted in antibacterial properties against oral pathogens.

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二氧化硅-羟基磷灰石共轭高氧化铈纳米粒子对正畸托槽的抗菌和细胞活力效应的演变

纳米材料因其不同于传统抗生素的作用机制而被用作抗菌剂。氧化铈纳米粒子（CeO2 NPs）对健康细胞的毒性极低，而且其独特的抗菌机制涉及 Ce（III）/Ce（IV）两种价态之间的可逆转变，因此在抗菌剂中发挥着重要作用。在本研究中，二氧化硅微球（SiO2）被用于羟基磷灰石（Hap）在二氧化硅表面的生长。然后，将二氧化硅/羟基磷灰石（SiO2/Hap）纳米复合材料用于在其表面稳定原位绿色合成的氧化铈纳米粒子（CeO2 NPs），以评估正畸托槽的抗菌性和细胞活力。通过傅立叶变换红外光谱、X射线衍射、FE-SEM、TEM和ICP-OES分析等不同技术对制备的SiO2/Hap-CeO2 NPs进行了全面表征。采用静电喷雾辅助气相沉积法为正畸带涂覆 SiO2/Hap-CeO2 NPs 纳米复合材料。采用生物膜抑制试验评估了 SiO2/Hap-CeO2 NPs 纳米复合材料对白色念珠菌、变异链球菌和嗜酸乳杆菌的抗菌效果。在近期研究的细胞和分子部分，使用 SiO2/Hap-CeO2 纳米复合材料处理的细胞通过 MTT 试验进行了评估，以确定对正常（HUVEC）细胞系的细胞毒性。与对照组相比，SiO2/Hap-CeO2 NPs 纳米复合材料组所有微生物的复制能力都下降了 2-log10。与对照组相比，SiO2/Hap-CeO2 NPs 纳米复合材料组的白喉杆菌、变异杆菌和嗜酸乳杆菌菌落数量明显减少。将 SiO2/Hap-CeO2 NPs 纳米复合材料应用于正畸带，可对口腔病原体产生抗菌作用。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.