Robot-based 6D bioprinting for soft tissue biomedical applications

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Franziska B. Albrecht, Freia F. Schmidt, Christian Schmidt, Rainer Börret, Petra J. Kluger
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Abstract

Within this interdisciplinary study, we demonstrate the applicability of a 6D printer for soft tissue engineering models. For this purpose, a special plant was constructed, combining the technical requirements for 6D printing with the biological necessities, especially for soft tissue. Therefore, a commercial 6D robot arm was combined with a sterilizable housing (including a high-efficiency particulate air (HEPA) filter and ultraviolet radiation (UVC) lamps) and a custom-made printhead and printbed. Both components allow cooling and heating, which is desirable for working with viable cells. In addition, a spraying unit was installed that allows the distribution of fine droplets of a liquid. Advanced geometries on uneven or angled surfaces can be created with the use of all six axes. Based on often used bioinks in the field of soft tissue engineering (gellan gum, collagen, and gelatin methacryloyl) with very different material properties, we could demonstrate the flexibility of the printing system. Furthermore, cell-containing constructs using primary human adipose-derived stem cells (ASCs) could be produced in an automated manner. In addition to cell survival, the ability to differentiate along the adipogenic lineage could also be demonstrated as a representative of soft tissue engineering.

Abstract Image

基于机器人的 6D 生物打印技术在软组织生物医学中的应用
在这项跨学科研究中,我们展示了 6D 打印机对软组织工程模型的适用性。为此,我们建造了一个特殊的设备,结合了 6D 打印的技术要求和生物学要求,尤其是对软组织的要求。因此,将商用 6D 机械臂与可消毒外壳(包括高效微粒空气过滤器和紫外线辐射灯)以及定制的打印头和打印平台结合在一起。这两个组件都可以进行冷却和加热,这对于处理有活力的细胞来说是非常理想的。此外,还安装了一个喷洒装置,可以喷洒细小的液滴。通过使用所有六个轴,可以在不平整或倾斜的表面上打印出先进的几何形状。基于软组织工程领域常用的具有不同材料特性的生物墨水(结冷胶、胶原蛋白和甲基丙烯酰明胶),我们可以展示打印系统的灵活性。此外,使用原代人类脂肪来源干细胞(ASCs)的含细胞构建体也可以自动生成。除了细胞存活外,还可以展示作为软组织工程代表的成脂系分化能力。
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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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