The duration-dependent and sex-specific effects of neonatal sevoflurane exposure on cognitive function in rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-20 eCollection Date: 2024-01-01 DOI:10.1590/1414-431X2024e13437

Jiangxia Cheng, Zhuo Wang, Hui Yu, Ye Chen, Zhengchao Wang, Liangcheng Zhang, Xiaohong Peng

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Abstract

Clinical studies have found that neonatal sevoflurane exposure can increase the risk of cognitive dysfunction. However, recent studies have found that it can exhibit neuroprotective effects in some situations. In this study, we aimed to explore the effects of sevoflurane neonatal exposure in rats. A total of 144 rat pups (72 males and 72 females) were assigned to six groups and separately according to sevoflurane exposure of different times on the seventh day after birth. Blood gas analysis and western blot detection in the hippocampus were conducted after exposure. The Morris water maze test was conducted on the 32nd to 38th days after birth. The expression of PSD95 and synaptophysin in the hippocampus was detected after the Morris water maze test. We found that neonatal exposure to sevoflurane promoted apoptosis in the hippocampus, and Bax and caspase-3 were increased in a dose-dependent manner. The 2-h exposure had the greatest effects on cognitive dysfunction. However, with the extension of exposure time to 6 h, the effects on cognitive function were partly compensated. In addition, sevoflurane exposure decreased synaptogenesis in the hippocampus. However, as the exposure time was extended, the suppression of synaptogenesis was attenuated. In conclusion, neonatal sevoflurane exposure exhibited duration-dependent effects on cognitive function via Bax-caspase-3-dependent apoptosis and bidirectional effects on synaptogenesis in rats.

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新生儿期七氟烷暴露对大鼠认知功能的影响与持续时间和性别有关。

临床研究发现，新生儿接触七氟烷会增加认知功能障碍的风险。然而，最近的研究发现，在某些情况下，七氟烷可以起到神经保护作用。在本研究中，我们旨在探索新生儿期接触七氟烷对大鼠的影响。我们将 144 只幼鼠（72 雄性和 72 雌性）分为 6 组，并在出生后第 7 天按照不同的七氟烷暴露时间分别进行分组。暴露后对海马进行血气分析和西部印迹检测。出生后第32至38天进行莫里斯水迷宫试验。莫里斯水迷宫试验后检测了海马中PSD95和突触素的表达。我们发现，新生儿暴露于七氟烷会促进海马的细胞凋亡，Bax和caspase-3的增加呈剂量依赖性。暴露2小时对认知功能障碍的影响最大。然而，随着暴露时间延长至 6 小时，对认知功能的影响得到了部分补偿。此外，七氟醚暴露会减少海马的突触生成。然而，随着暴露时间的延长，对突触生成的抑制作用有所减弱。总之，新生儿暴露于七氟烷会通过依赖于Bax-caspase-3的细胞凋亡和对突触生成的双向影响对大鼠的认知功能产生持续时间依赖性的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.