Histological and biochemical effects of an ethanolic extract of Myrtus communis leaf on the pancreases of rats fed high fat diets.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Gul Sinemcan Kabatas, Busra Ertas, Ali Sen, Goksel Sener, Feriha Ercan, Dilek Akakin
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Abstract

We investigated the effects of an ethanolic extract of Myrtus communis subsp. communis (MC) leaves on the pancreases of rats fed with a high fat diet (HFD). Wistar albino rats were fed either with standard lab chow (Control group) or with a 45% fat diet (HFD and HFD+MC groups) for 4 months, with the MC extract (100 mg/kg) being administered by orogastric gavage to rats in the HFD+MC group during the last month. Blood and pancreas samples were collected from all experimental groups at the end of the study. Insulin and leptin levels, and the lipid profile, were analyzed in the blood serum. Pancreatic injury was assessed histologically. Insulin, nuclear factor kappa beta (NF-κB), and alpha-smooth muscle actin (α-SMA) were assessed using immunohistochemistry. Apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) immunohistochemistry. In addition, oxidant/antioxidant activity was analyzed by biochemical methods. Increased body weight, serum insulin and leptin levels, blood glucose level and pancreatic tissue malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels, myeloperoxidase (MPO) activity and decreased tissue glutathione (GSH) level were observed in the HFD group compared to the Control group, in addition to dyslipidemia. An increased histopathological damage score, pancreatic islet area, insulin, TUNEL, NF-κB and α-SMA immunoreactivity were seen in animals from the HFD group compared to the Control group. However, such pathological changes were reduced in the HFD+MC group. Our data indicate further investigation of MC extract as a therapeutic adjuvant for HFD-induced pancreatic injury, acting via anti-inflammatory and antioxidant mechanisms, is worth carrying out.

香桃木叶乙醇提取物对高脂饮食大鼠胰腺的组织学和生化影响
我们研究了香桃木亚种(MC)叶乙醇提取物对高脂饮食(HFD)大鼠胰腺的影响。Wistar 白化大鼠以标准实验室饲料(对照组)或 45% 脂肪饮食(HFD 组和 HFD+MC 组)喂养 4 个月,在最后一个月给 HFD+MC 组大鼠灌胃 MC 提取物(100 mg/kg)。研究结束时收集所有实验组的血液和胰腺样本。对血清中的胰岛素和瘦素水平以及脂质概况进行分析。对胰腺损伤进行组织学评估。胰岛素、核因子卡巴β(NF-κB)和α-平滑肌肌动蛋白(α-SMA)通过免疫组化进行评估。凋亡采用末端脱氧核苷酸转移酶 dUTP 缺口末端标记(TUNEL)免疫组化法进行评估。此外,还采用生化方法分析了氧化剂/抗氧化剂活性。与对照组相比,HFD 组的体重、血清胰岛素和瘦素水平、血糖水平、胰腺组织丙二醛 (MDA)、8-羟基-2'-脱氧鸟苷 (8-OHdG) 水平、髓过氧化物酶 (MPO) 活性增加,组织谷胱甘肽 (GSH) 水平降低,此外还有血脂异常。与对照组相比,HFD 组动物的组织病理学损伤评分、胰岛面积、胰岛素、TUNEL、NF-κB 和 α-SMA 免疫反应活性均有所增加。然而,这些病理变化在 HFD+MC 组中有所减少。我们的数据表明,值得进一步研究 MC 提取物通过抗炎和抗氧化机制作为 HFD 诱导的胰腺损伤的治疗辅助剂。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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