Correlation between biomedical and structural properties of Zn/Sr modified calcium phosphates

IF 4.1 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biometals Pub Date : 2024-05-28 DOI:10.1007/s10534-024-00599-w

Atipong Bootchanont, Natthaphon Chaosuan, Sasina Promdee, Jantima Teeka, Pinit Kidkhunthod, Rattikorn Yimnirun, Wutthigrai Sailuam, Nutthaporn Isran, Arreerat Jiamprasertboon, Theeranun Siritanon, Tanachat Eknapakul, Thanit Saisopa

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Abstract

This study investigates the correlation between the biomedical and structural properties of Zn/Sr-modified Calcium Phosphates (ZnSr–CaPs) synthesized via the sol–gel combustion method. X-ray diffraction (XRD) analysis revealed the presence of Ca₁₀(PO₄)₆(OH)₂ (HAp), CaCO₃, and Ca(OH)₂ phases in the undoped sample, while the additional phase, Ca₃(PO₄)₂ (β-TCP) was formed in modified samples. X-ray absorption near-edge structure (XANES) analysis demonstrated the incorporation of Sr into the lattice, with a preference for occupying the Ca1 sites in the HAp matrix. The introduction of Zn, furthermore, led to the formation of ZnO and CaZnO₂ species. The ZnSr–CaPs exhibited significant antibacterial activity attributed to the generation of reactive oxygen species by ZnO, the oxidation reaction of CaZnO₂, and the presence of Sr ions. Cytotoxicity tests revealed a correlation between the variation in ZnO content and cellular viability, with lower ZnO concentrations corresponding to higher cell viability. Additionally, the cooperative effects of Zn and Sr ions were found to enhance the bioactivity of CaPs, despite ZnO hindering the apatite formation process. These findings contribute to the deep understanding of the diverse role in modulating the antibacterial, cytotoxic, and bioactive properties of ZnSr–CaPs, offering potential applications in the field of biomaterials.

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Zn/Sr 改性磷酸钙的生物医学特性与结构特性之间的相关性。

本研究探讨了通过溶胶-凝胶燃烧法合成的 Zn/Sr 改性磷酸钙（ZnSr-CaPs）的生物医学特性与结构特性之间的相关性。X 射线衍射（XRD）分析表明，未掺杂样品中存在 Ca10(PO4)6(OH)2 (HAp)、CaCO3 和 Ca(OH)2 相，而改性样品中形成了额外的 Ca3(PO4)2 (β-TCP) 相。X 射线吸收近边结构（XANES）分析表明，Sr 被掺入了晶格中，并优先占据了 HAp 基体中的 Ca1 位点。此外，锌的引入导致了氧化锌和 CaZnO2 物种的形成。ZnSr-CaPs 具有显著的抗菌活性，这归因于 ZnO 产生的活性氧、CaZnO2 的氧化反应以及 Sr 离子的存在。细胞毒性测试表明，氧化锌含量的变化与细胞存活率之间存在相关性，氧化锌浓度越低，细胞存活率越高。此外，尽管氧化锌阻碍了磷灰石的形成过程，但锌离子和锶离子的协同作用增强了 CaPs 的生物活性。这些发现有助于深入了解 ZnSr-CaPs 在调节抗菌、细胞毒性和生物活性特性方面的不同作用，为生物材料领域提供了潜在应用。

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来源期刊

Biometals 生物-生化与分子生物学

CiteScore

5.90

自引率

8.60%

发文量

111

审稿时长

3 months

期刊介绍： BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.